Detection Of Promethazine And Amikacin Based On The Room Temperature Phosphorescence Of MPA-capped Mn-doped ZnS Quantum Dots

Nowadays,promethazine and amikacin are more and more used in clinical research,but both of them will also have a certain pharmacological toxicity to human or animal while being used in curing diseases.Therefore,establishing a rapid and effective detection method of promethazine and amikacin is imminent.Recently,quantum dots(QDs)have been widely used in chemical,electronics and biomedical fields as a novel inorganic nanomaterial with excellent features of optics,electronics and surface modification,it has gradually become a new fluorescent material with better prospect than organic dyes.Compared with organic small molecular dyes,quantum dots have narrow and symmetrical emission spectra,excellent light stability and other optical properties.Hence,quantum dots as fluorescent probes are widely used in the detection of various ions,small molecules and biological macromolecules.However,the fluorescent quantum dots are susceptible to interference of biological fluid background fluorescence,whereas the phosphorescent quantum dots do not.Based on this,a new method for the detection of promethazine and amikacin was established based on the room temperature phosphorescence of MPA capped Mn doped ZnS quantum dots.The main results are as follows:(1)MPA capped Mn doped ZnS quantum dots were prepared by aqueous phase synthesis method using trimercapto propionic acid(MPA)as stabilizer.The synthesized quantum dots were characterized by XRD and phosphorescence spectroscopy.The results show that the prepared QDs have a zinc blende structure,the maximum excitation wavelength of RTP is295 nm,and the maximum emission wavelength is 590 nm.(2)A new detection of promethazine was established based on the room temperature phosphorescence of MPA capped Mn doped ZnS quantum dots.In phosphate-buffered saline solution,the addition of promethazine could obviously quench the room temperature phosphorescence of MPA capped Mn doped ZnS quantum dots.The quenched phosphorescence intensity amount(?RTP)was linearly proportional to the concentration of promethazine.The linear relationship was divided into two sections: ranged from 3.2 to 32?mol/L and 32 to 160 ?mol/L,which the regression coefficient was 0.998 and 0.999 respectively.The limit of the detection method was 0.553 ?mol/L.The method can avoid the interference of autofluorescence and scattering light in the biological fluid,and does not need to add any deoxidizer and inducer.This method could detect promethazine in human serum and urine samples with satisfying results,and the recovery ranged from 96.4% to 103.1%.(3)A new method for the detection of amikacin was established based on the room temperature phosphorescence of MPA capped Mn doped ZnS quantum dots.The system was in phosphate-buffered saline solution(PBS,pH7.4,0.1M)reacted at room temperature for 10 min.The RTP of MPA capped Mn doped ZnS QDs could be remarkably enhanced with the addition of amikacin.Under the optimized experimental conditions(the concentration of the QDs was 2mg mL-1),the method showed a good linearity(regression coefficient = 0.997)in the concentration range of 0.2-6?M for amikacin,and the limit of the detection method was0.085?M.Since amikacin was more used to treat cow breast disease,the proposed method was successfully applied to detect amikacin in milk,human serum and urine samples with satisfying results,and the recovery ranged from 96.2% to 101.2%.