Determination Of Trace Hormones By Solid Substrate Room Temperature Phosphorimetry And The Forecast Of Human Diseases

This dissertation focused on the development of new phosphorescent labelling reagents. Based on the combination between high sensitivity of solid substrate room temperature phosphorimetry （SSRTP） and high specificity of bio-reaction, SSRTP immunoassay （SSRTPIA） for the determination of important hormones in human body, the feasibility of the prediction of human diseases and the mechanism of the methodology were explored, showing wide application prospect of SSRTPIA.In Chapter One, the research progress, potential advantages, limitations, researching focus and the developing trends of SSRTP （including catalytic SSRTP, ion association SSRTP, SSRTPIA and affinity adsorption SSRTP） were reviewed, the applications in inorganic chemistry and biology were summarized. Meanwhile, the developing trends and application were prospected. The idea of this dissertation was proposed based on considerable references.In Chapter Two, a new water soluble MWNTs （expressed as MWNTs’） was developed as phosphorescent labelling reagent for the first time, based on the strong oxidation effect of （C₆H₅CO）₂O₂ on cutting the tail of MWNTs into -COOH group.Theβ-human chorionic gonadotrophin （HCG） antibody （AbHCG） labelled by MWNTs’to get the product of MWNTs’-AbHCG, which could take specific quantitative immune reaction with HCG. Thus, new SSRTPIA was developed for the determination of HCG and the diagnosis of human diseases. And the results coincide well with chemiluminescence immunoassay of Zhangzhou Municipal Hospital of Fujian Province. The mechanisms for the determination of HCG and for MWNTs’labelling AbHCG were also discussed.In Chapter Three, Based on the–COOH group of MWNTs’reacted with the–NH2 group of Rhodamine S （Rhod. S） to produce Rhod. S-MWNTs’, which reacted with Tween-80 to form micelle compound Tween-80-Rhod. S-MWNTs’, which could be used as phosphorescent labelling reagent was developed. Tween-80-Rhodamine S-MWNTs’-AbCT was obtained by Tween-80-Rhod. S-MWNTs’labelling antibody of calcitonin （AbCT）. Making use of the high specificity of immune reaction between Tween-80-Rhodamine S-MWNTs’-AbCT and CT, a new SSRTPIA was developed for the determination of CT using Tween-80-Rhodamine S-MWNTs’micelle compound. Simultaneously, the analytical application and the mechanism for the determination of trace CT were discussed.In the Chapter Four, phosphorescent labelling reagent CdTe quantum dots （QDs） and phosphorescent sensor CdTe-QDs-AbGAS （AbGAS was antibody of gastrin） for gastrin （GAS） were developed. Based on the high specific reaction between CdTe-QDs-AbGAS and GAS, a new SSRTPIA was established for detecting GAS using CdTe-QDs-AbGAS as phosphorescent sensor. The determination of GAS in biological samples and the diagnosis of human diseases, as well as the reaction mechanism were also investigated.In Chapter Five, the main research results of this paper were summarized, and their application prospect was prospected.