| Purpose:The purpose of this paper is to study the separation and purification of petroleum ether extract of Stellera chamaejasme,determinein the anticancer activity of the isolation in vitro,and find the active ingredient that exhibit anti-cancer activity.Methods:Stellera chamaejasme was extracted with petroleum ether by methods of impregnation at room temperature.Then the petroleum ether extract was separated and purified by silica gel column chromatography.The structures of the isolation was identified by mass spectrometry and nuclear magnetic resonance techniques.Human embryonic lung fibroblasts MRC-5,human lung cancer cell NCI-H226,highly metastatic lung cancer cell 95D and the human hepatoma cell BEL-7404 were treated with the isolation with different concentrations.The toxic effect of the isolation on the four cells was determined by MTT assay.Results:1.A monomer compound,neochamaejasmine A,was isolated from the petroleum ether extract of Stellera chamaejasmel.2.All of the four cells are inhibited by neochamaejasmine A.In 24h,the maximum inhibition rate of neochamaejasmine A with different concentrations on MRC-5 cells,NCI-H226 cells,95D cells,and BEL-7404 cells were 55.77%,54.49%,34.50%and 73.54%,respectively.In 48h,the maximum inhibition rate of neochamaejasmine A with different concentrations on MRC-5 cells,NCI-H226 cells,95D cells,and BEL-7404 cells were 83.34%,54.49%,55.52%and 87.14%,respectively.IC50 values of the 4 cells in 24h were 85.59μg/mL,94μg/mL and 47.05μg/mL,respectively.IC50 values of the 4 cells in 4h were 56.55μg/mL,75.39μg/mL,88.87μg/mL and 28.1μg/mL,respectively.3.The toxic effect of neochamaejasmine A on BEL-7404 cells was the strongest,followed by MRC-5 cells,NCI-H226 cells and 95D cells.4.The toxic effect of neochamaejasmine A on cancer cells exhibit a certain selectivity.The toxic effect of neochamaejasmine A on liver cancer cells was significantly stronger than that of lung cancer cells.Conclusions:Neochamaejasmine A maybe one of the active ingredients from petroleum ether extrac that exhibit anti-cancer activity. |
