| Objective:The aim of this study was to investigate the association of 15 SNPs with androgenetic alopecia by analyzing the differences of 15 SNPs loci polymorphisms between androgenetic alopecia case group and healthy control group in the YunnanHan population.Methods:According to the diagnostic criteria of Norwood/Hamilton,115 cases ofHan male patients who were diagnosed as AGA were recruited from the First Affiliated Hospital of Kunming Medical University.Prticipants with other diseases and smoking and alcohol abuse were exluded.Correspondingly,128 fealthy males were selected as the control group.The genomic DNA of the whole blood was extracted by phenol-chloroform method.7 SNPs and 8 SNPs co-amplification protocol were established,and adopting SNaPshot platform detected the genotype of the 15 SNPs for all the subjects.The statistical analysis for HWE values,allele frequencies,genotypic association analysis,linkage disequilibrium analysis,haplotype analysis,and interaction between loci and loci at each locus were performed using Plink1.07 software,SNPAnalyzer2.0 software,Haploview4.2 software and GMDR0.9 software package.Results:1 Association of single polymorphismThe allele frequencies,codominant genotype frequencies and frequencies of dominant genotype of rs929626 and rs7975017 were significantly different between the case and the control group(P<0.01).Only one genotype of rs1562438 was detected excluded in the subsequent statistical analysis.There were no significant differences in allele frequencies,codominant genotype frequencies and dominant genotype frequencies of the other 12 SNPs loci between the AGA case group and the healthy control group(P>0.05).The genotype frequencies of the recessive genetic patterns of the 14 SNPs loci were not statistically different between the androgenic alopecia patients and the healthy controls.2 Linkage disequilibrium analysisIn males of Yunnan Han population,there was weak linkage disequilibrium between rs10193725?rs7349332(Chr2q35);rs7648585?rs4679955(Chr3q25.1);rs929626?rs1081073(Chr5q33.3);rs9668810?rs7975017(Chr12pl2.1)and rs7950226?rs11022775?rs11022778(Chr11p15.3)in AGA case group and the control group(D'<0.8)but strong linkage disequilibrium between 3 SNPs of Chr11p5.3 in AGA group and the control group.3 The haplotype analysisThrough haplotype analysis of 11 SNPs(Chr2q35:rs10193725?rs7349332;Chr3q25.1:rs7648585?rs4679955;Chr5q33.3:rs929626?rs1081073;Chr11p15.3:rs7950662?rs11022775?rs11022778;Chr12pl2.1:rs9668810?rs7975017)in the males of Yunnan Han population,there were significant haplotype frequency distribution differences between the two groups in haplotypes of h3(T-A)constructed with rs7648585-rs4679955 on Chr3q25.1,haplotype of h2(G-A)?h4(A-T)constructed with rs929626-rs1081073 on Chr5q33.3 and haplotype of h3(T-T)?h4(C-T)constructed with rs9668810-rs7975017 on Chr12P12.1(P<0.01).Moreover,the h2(G-A)haplotypes constructed with rs969626-rs1081073 was different in the two groups,P = 2.50E-04,OR = 2.127,95%CI=1.415-3.198.4 The GMDR analysisThe results of GMDR analysis showed that the optimal interaction model was rs7975017-rs2497938 two factor interaction model(CVC 10/10,Training balance accuracy:71.69%,Testing balance accuracy:70.46%)which was associated with androgenetic alopecia(P = 0.001).Conclutions:(1)The polymorphisms of rs929626 and rs9795017 are associated with AGA in Yunnan Han population.And the individual with C allele of rs7975017 or AA genotype of rs929626 may be easier to suffer from androgenetic alopecia.(2)Individual with rs969626-rs1081073 haplotype G-A may increase the probability of suffering from androgenic alopecia.(3)There is interaction among rs7975017and rs2497938 increasing the probability of suffering from androgenic alopecia. |
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