| BackgroundSchizophrenia is a complex clinical syndrome,and its etiology has not been fully elucidated by researchers.The hypothesis of immune inflammation is one of the most important theories in the study of schizophrenia,which is based on the increased levels of inflammatory cytokines in peripheral blood(Immune activation).In the human body,the common inflammatory cytokines include high sensitivity C-reactive protein(hs-CRP),transforming growth factor beta 1(TGF-beta 1),interleukin 1 beta(IL-1 beta),interleukin 6(IL-6)and interleukin 17(IL-17).Studying the changes of inflammatory cytokines before and after treatmentin human body,and comparing the differences between them in different groups of patients is helpful to clarify the influence of immune inflammation on the prognosis of schizophrenia.Objectives1.To observe and explore the change rules of peripheral inflammatory cytokines before and after treatmentin first-episode schizophrenic patients in order to enrich the clinical evidences of schizophrenia immune inflammation hypothesis.2.Compare the differences of inflammatory cytokines in patients with different clinical efficacy,and analyze the clinical significance of the differences in order to obtain the possibility of evaluating potential biological indicators of clinical efficacy of schizophrenia.Methods1.82 first-episode schizophrenic patients in according with diagnostic criteria for schizophrenia in line with the tenth of international classification of diseases(ICD-10)were choiced and used olanzapine for 4 weeks singly.Positive and negative symptom scale(PANSS)scores were assessed before and 4 weeks after treatment.The patients were divided into A and B group according to the PANSS reduction rate(50%).At the same time,30 healthy subjects matched for age and sex were selected as control group.2.Draw peripheral venous blood 5ml at 6:30-7:30 in the morning before and 4 weeks after treatment,centrifuge and package the upper plasma,store at-70?.Enzyme linked immunosorbent assay(ELISIA)was used to detect the levels of TGF-beta 1,IL-1 beta,IL-6 and IL-17 in serum of patients,and Latex enhanced immunoturbidimetry to hs-CRP.3.SPSS 19 was used to deal with the datas,the measurement datas were expressed as mean ± standard deviation,and adopped the paired samples t test or independent sample t test,P < 0.05 showed statistical difference.Result1.The PANSS score of 82 patients was 88.34+11.50 before treatment anddecreased to 67.15+18.69 after treatmen.The reduction rate of PANSS of 42 of thepatients was less than 50%(A group)and 40 of the patients was more than 50%(groupB).2.Before treatment,the levels of 5 kinds of inflammatory factors in patients groupwere significantly higher than that in healthy controls,the difference was statisticallysignificant(P<0.05).The level of hs-CRP increased significantly after 4 weeks of treatment,the difference was statistically significant(P< 0.05).The level of TGF-beta 1 in no much difference before and after treatment(P 0.05).The level of IL-1 beta,IL-6 and IL-17 were significantly reduced,the differences were statistically significant(P < 0.05).3.After 4 weeks of treatment,the level of hs-CRP was significantly higher than before treatment in 2 groups in the serum of the patients,the differences were statistically significant(P< 0.05).Compared with before treatment,the levels of IL-1,IL-6 and IL-17 were significantly decreased,the differences were statistically significant(P< 0.05).The levels of IL-1,IL-6 and IL-17 in serum of patients in group A were lower than those in group B after treatment,the differences were statistically significant(P< 0.05).Conclusions1.There is an abnormality of immunity in schizophrenia,and olanzapine can improve this abnormality.2.The levels of serum hs-CRP and TGF-?1 in schizophrenic patients may not be an inflammatory marker to evaluate the clinical efficacy of schizophrenia.3.IL-1?,IL-6 and IL-17 can be used as potential biological indicators to evaluate the clinical efficacy of patients. |
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