| Background:Spinal cord injury(SCI)is one of the most serious injuries in the production and life.With the rapid development of the industrial age,the number of SCI patients is increasing year by year due to traffic accidents,sports injuries and falling,mostly in young adults.After spinal cord injury,the regeneration ability of neurons is weak,and it is often easy to cause paraplegia.The treatment effect is not ideal.So far,people have not found a good way to solve this problem.It is one of the ideal methods to treat spinal cord injury in the early stage of spinal cord decompression and internal fixation combined with traction,hyperextension reduction and fracture dislocation,interbody fusion and internal fixation.Drug treatment with Methylprednisolone pulse therapy,but the treatment effect is not ideal.herefore,we have been exploring some new techniques applied to effectively solve the problem of weak regeneration ability of neurons after spinal cord injury,the axons can regenerate,spinal cord function can be reconstructed,and the new techniques can be applied to clinical treatment as soon as possible.In recent years,with the development of tissue engineering technology research and development and further research on the pathological changes and mechanism of SCI,scientists have proposed using stem cell gene therapy for the treatment of spinal cord injury ideas,provides a new method for the treatment of spinal cord injury.Objective:Aiming to explore the possible mechanism of bone marrow mesenchymal stem cells transplantation for spinal cord injury,and to provide new ideas and methods for the treatment of spinal cord injury.Method:1 The bone marrow mesenchymal stem cells were isolated and cultured by whole bone marrow adherent culture method.2 by adenovirus vector in vitro transfection of Shootinl into BMSC,the expression of Shootinl protein was detected in 3;in vitro differentiation of neural cells,observe whether differentiate into neuron like cells;4 to establish a rat model of spinal cord injury repair in the treatment of impact,within half an hour at Shootinl-BMSC and BMSC respectively in situ transplantation for spinal cord injury a week after spinal cord injury,BBB scores on motor function of rats,the rats were killed after 5 weeks,the spinal cord specimens were frozen,immunofluorescence assay related neuronal marker protein.Result:1 Compared with non transfected Shootinl bone marrow mesenchymal stem cells,Shootinl lentivirus infected BMSCs were stable and high level expression of Shootinl protein in vitro;2 neural differentiation after 7 days,Shootinl transfected BMSC and normal BMSC cell morphological change,we detected by IF neural precursor cells marker Nestin and neuron specific nuclear protein NeuN,expression of Shootinl-BMSC and BMSC there is no significant difference between two groups of Nestin and NeuN protein;3 bone marrow mesenchymal stem cell transplantation,motor function score,compared with group PBS cell transplantation group,the recovery of motor function has obvious changes,and the Shootinl-BMSC group BBB score was the highest,but the Shootinl-BMSC transplantation group and BMSC group were not statistically significant compared.Conclusion:1 This experiment proved that BMSCs cell transplantation can effectively promote the recovery of spinal cord motor function after injury;2 this experiment showed that Shootinl protein can promote nerve regeneration and functional region after spinal cord injury recovery,but the effect is directly BMSCs transplantation group is not obvious,we need to further explore the mechanisms of bone marrow mesenchymal stem cells transplantation for the treatment of spinal cord injury. |
PDF Full Text Request