| ObjectiveTo investigate the effect of stable SOD gavage on nonalcoholic fatty liver and glucose metabolism in mice and the possible mechanism.Methodexperiment 1:The C57BL/6J mices were randomly divided into NC group,HFD group and SOD group.Mices in NC group was given basic chow,and the other groups were fed with high fat diet.At the eighth week,mices in NC group and HFD group were gavage with sterile water and the SOD group was given MS-SOD solution gavage.Gavage was 1 times a day until the end of the experiment.Glucose metabolism was measured before the end of the experiment.At the end of the experiment,the visceral tissues were preserved and tested.Test projects including the fatty liver,liver immune factors,intestinal barrier,metagenomic and microbiota.Experiment 2:The C57BL/6J mices were randomly divided into high fat group,antibiotics high fat group and antibiotics high fat SOD group.Three groups of mice were fed with high fat diet,and mices in the latter two groups were treated with antibiotics.At the second week of experiment,the mices in the SOD group were gavaged with MS-SOD solution,and the other groups were gavaged with sterile water until the experiment ends.Glucose metabolism was measured before the end of the experiment.At the end of the experiment,the visceral tissues were preserved and tested.Test projects including the fatty liver and liver immune factors.Experiment 3:The ob/ob mices were randomly divided into control group and SOD group.After feeding for 4 weeks,mices in the control group was given sterile water and the mice in group SOD were given MS-SOD solution gavage 1 times a day until the end of the experiment.Glucose metabolism was measured before the end of the experiment.At the end of the experiment,we collect the splanchnic tissue and doing the test.The test items include liver HE,oil red staining and liver immune factor,fecal albumin and intestinal microflora.ResultExperiment 1:1,the expression of Mn-SOD in intestinal bacteria was decreased in HFD group.2,SOD group mices’ fasting blood glucose,GTT test and other glucose metabolism test is better than HFD mices.3,SOD group mices’ fatty liver score were lower than HFD group mices.The ccl4,ccl8 and etc inflammatory factor mRNA expression in liver of SOD group was lower than that in HFD group.4,NC group,HFD group and SOD group show the trend of differentiation in the PCOA chart which can reflect the diversity of the microbiota.5,SOD group mices’intestinal tight junction protein detection and fecal albumin were better than that of HFD group.Experiment 2:The sugar metabolism,fatty liver and liver inflammatory factors in the mices of high fat group were lower than those in antibiotics high fat group and antibiotics high fat SOD group,but there was no significant difference between the latter two groups.Experiment 3:1,SOD group mices’GTT test and other sugar metabolism was better than the control group;2,SOD group mices’ fatty liver in mice was superior to the control group.The ccl4 inflammatory factor mRNA expression in liver cell of SOD group was lower than that in the control group;3,it shows the distinguish trend between SODgroup and control group in PCOA diagram which can reflect the beta diversity of intestinal flora.4,SOD group mices’ feces albumin was lower than the control group;ConclussionExperiment 1:High fat diet resulted in Mn-SOD expression in mices’ intestinal microbiota;MS-SOD gavage administration can effectively alleviate the symptoms of glucose metabolism abnormal and nonalcoholic steatohepatitis in mice fed with high fat diet,and the possible mechanism is that MS-SOD can change the microbiota and protect intestinal barrier.Experiment 2:MS-SOD did not further promote glucose metabolism and fatty liver under the effect of antibiotics,and it proved that the acction site of SOD is related to intestinal flora.Experiment 3:SOD can alleviate the abnormal of glucose metabolism and nonalcoholic fatty liver disease in ob/ob mice.The possible way was MS-SOD can change the microbiota and protect intestinal barrier. |
