Effect Of TBCB On Ischemia-Reperfusion Injury In Mice Liver

Objective: This experiment through the establish a model of hepatic ischemia-reperfusion injury(IRI)in mice,and to investigate the expression of tubulin folding factor B(TBCB)at different time points after hepatic ischemia-reperfusion in mice.It is the role of liver ischemia-reperfusion injury in mice and its relationship.n injuryMethods:1.The mouse liver block ischemia-reperfusiomodel we selected 60 healthy C57 mice were randomly divided into control group(n = 7)and ischemia-reperfusion group(n = 42).The ischemia-reperfusion group was divided into two groups: ischemia-reperfusion group,ischemia-reperfusion group(2,4,6,8,12,24 h)were divided into 6 subgroups,7 subgroups of each subgroup,and the mice were sacrificed by total hepatic ischemia Reperfusion injury model,respectively,after the end of the experiment to remove the mouse blood and liver tissue.2.The expression levels of ALT and AST in serum were measured by automatic biochemical analyzer.The expression levels of IL-6 and TNF-? in serum was detected by ELISA.3.Morphological changes of liver were observed by HE staining.The expression of TBCB protein,IL-6 and TNF-in liver tissue at different time points of ischemia were detected by Western Blot and immunohistochemistry.4.Real-Time PCR was used to detect the mRNA expression and changes of TBCB in liver tissue at different time point in ischemia reperfusion group.Results:1.The levels of serum IL-6 and TNF-? in serum and serum ALT and AST were detected by automatic biochemical analyzer.Compared with the control group,the levels of AST,ALT and IL-6 in ischemia reperfusion group(P <0.05).The expression of TNF-? was significantly higher than that of the control group(P <0.05),and there was significant difference between the two groups at the time of reperfusion(P <0.05).2.HE staining was used to observe the morphological changes of the liver.After we found that the ischemia reperfusion group compared with the control group,the liver morphology changed significantly,especially at 12 h after ischemia reperfusion,the liver cell degeneration and necrosis were obvious,the liver structure disappeared,Partial hepatocellular focal necrosis3.The expression of TBCB,IL-6 and TNF-? in the control group and the ischemia-reperfusion group were detected by immunohistochemistry.The results showed that TBCB,IL-6 and TNF-? The expression of TBCB,IL-6 and TNF-? protein reached the peak at 12 h,and the expression level of TBCB,IL-6and TNF-? protein reached the peak at 12 h in the ischemia reperfusion group at different time points.4.Real-Time PCR and Western Blot showed that the expression of TBCB and TBCB protein was significantly higher than that of the control group,andthe change was the most obvious at 12 h in the ischemia reperfusion group.5.The results of correlation analysis showed that the expression of TBCB protein was correlated with the levels of TNF-and IL-6 protein in liver ischemia-reperfusion(P < 0.05)Conclusions:1.The expression of TBCB in liver tissue was significantly higher than that in sham operation group at the level of gene and protein level,which indicated that TBCB might be involved in hepatic ischemia-reperfusion injury A target.2.Our study shows that TBCB expression in ischemia-reperfusion injury,and IL-6,TNF-? in mice liver ischemia-reperfusion expression,expression was a certain correlation,TBCB and IL-6,TNF-? plays a key role in the occurrence of liver injury during hepatic ischemia-reperfusion.