Association Of Single Nucleotide Polymorphism (SNPs) Of ANGPTL2 Gene With Infarct-related Artery Spontaneous Reperfusion

Objective: To investigate the association of single nucleotide polymorphism of ANGPTL2 gene with infarct-related artery spontaneous reperfusion in patients with ST-segment elevation myocardial infarction(STEMI).Methods: The genomic DNA was extracted from the peripheral blood of 36 cases of spontaneous reperfusion(SR)patients and 62 cases of non-spontaneous reperfusion(NSR)patients,the five exons(Exon1-5)and three SNPs(rs999092(A>G),rs11792699(A>G)and rs13298677(A>G))of ANGPTL2 gene were amplified with fluorescence quantitative PCR,and detected by Sanger sequencing,the results were compared with the standard sequence of UCSC gene library,to analysis whether there is gene mutation in each exon,also the difference of SNPs locus genotype and allele frequencies in two groups.Results: The uric acid,WBC and mean platelet volume(MPV)in SR group was lower than that of NSR group,Exon1-3 were not detected with gene mutation,Exon4 has 48C>T heterozygous mutation,expression differences between the groups,Exon5 has two heterozygous mutations 271T>C and 587G>A,but no significant difference between the groups,the genotype and allele frequencies in 3 SNPs has no significant difference in the groups.Logistic regression analysis showed that uric acid(OR=1.009,95%CI: 1.002-1.016,P=0.015)and MPV(OR=2.206,95%CI: 1.221-3.986,P=0.017)were independent predictors of SR,and negatively correlated with the occurrence of SR.Conclusion: Exon4-5 exists gene mutation,and Exon4 48>T has significant differences in patients with SR and NSR,and may be caused the differences in the expression also function of ANGPTL2 protein,and has a correlation with the occurrence of SR.