Neuroprotective Effect Of ?-1,3-Galactosyltransferase?on Neurodegeneration Via Mediating Notch Signaling Pathway

Objective Our study was to determine the effect of ?-1,3-galactosyltransferase II on senescence and soluble A?1-42-mediated neurodegenerative changes and its effect on Notch1,to clarify ?-1,3-galactosyltransferase ? in neurodegenerative changes and its mechanism.Our results will provide a theoretical basis for further study glycosyltransferase on age-related neurodegenerative diseases for prevention,diagnosis and treatment..Methods Animals group: Mice were divided into two groups,the normal aged model mice(24 months old)and the young control group(3 months old).The expression of ?-1,3-galactosyltransferase ? protein,neuron dendritic marker MAP2 and Notch signaling pathway were detected by immunohistochemical staining and Western Blotting.Cells group: Cortical cells were prepared from 17 days CD-1 mice embryonic cerebral cortex and were randomly divided into six groups,control group;A?1-42 group;?-1,3-galt2 c DNA group;A?1-42 + ?-1,3-galt2 c DNA group;?-1,3-galt2 si RNA group;A?1-42 + ?-1,3-galt2 si RNA group.RT-PCR and immunohistochemical staining were used to verify the effect of ?-1,3-galactosyltransferase ? c DNA and si RNA transfection.Immunohistochemistry was used to observe the expression of MAP2 and the expression of SNAP25 and Notch1 in the neurons of?-1,3-galactosyltransferase ? overexpression and low expression.Results 1.?-1,3-galt2 expression was decreased in the elderly cerebral cortex.2.Notch1 expression was decreased in the elderly mice cerebral cortex.3,Soluble A?1-42 reduce the expression of ?-1,3-galt2 gene and protein in cortex cells,and also reduce the expression of Notch1.4,?-1,3-galt2 overexpression can improve the effect of soluble A?1-42 on dendritic marker protein MAP2 and synaptic related protein SNAP25.5,?-1,3-galt2 overexpression or low expression can respectively improve or aggravate the effect of soluble A?1-42 on Notch1.Conclusions 1,?-1,3-galactosyltransferase ? plays an important role in senescence and soluble A?1-42-mediated neurodegenerative changes.2,?-1,3-galactosyltransferase ? may play its role in neurodegenerative changes in neuronal protection through the Notch signaling pathway.