The Effect Of Ang-(1-7) And A779 On Human Hepatocellular Carcinoma Cell HUH-7 Cultured In Vitro Via The G Protein-coupled Receptor MAS

Objectives: To explore the effect of Ang-(1-7) and A779 in hepatocellular carcinoma(HCC) of Huh-7 cells which are cultured in vitro in terms of the proliferation, apoptosis and cell cycle .To invagation the expression of Mas by using drug Ang-(1-7) and A779 respectively in different situation.(3)To detect the effect of drug Ang-(1-7) and A779 in hepatocellular carcinoma(HCC) of Huh-7 cells in invasive and metastasis ability. (4) To discuss the effect of Ang-(1-7) and A779 through Mas in hepatocellular carcinoma(HCC) of Huh-7 cells.Methods: (1) Conducting RT-PCR to detect the expression level of Mas when treated with Ang(1-7) and A779 .(2) Using flow cytometry instrument to detect changes of apoptosis and cell cycle when treated with Ang(1-7) and A779 respectively . (3)Using wound healing and Transwell to test cell invasive ability when treated with Ang(1-7) and A779 .Results: (1) when treated with Ang-(1-7) and A779 receptively,the cellular morphology, the celluar structure and the cellular arrangement do not change significantly. (2) when reated with Ang-(1-7) separately, the apoptotic cell of Ang (1-7) compared with control group increased . The apoptosis ratio of Ang(1-7) group , A779 group and control were 95.2% 30.2%and 42.7% respectively. Ang- (1-7) group was significantly higher than control group (P<0.05) . A779 group was lower than control group (P<0.05). (3) In RT-PCR,the relative expression amount of Mas in Ang (1-7) group, A779 group and control group was 2.735 , 0.498 and 1 separately . Ang(1-7) group was higher than control group . A779 group was less than control group . The difference has statistically significant (P<0.05) . (4) The change of the celluar cycle was not obvious when treated with Ang(1 -7) and A779 independently . (5 )The healing rate of wounding healing experiment was 9.70% , 22..27% and 20.74% in Ang- (1-7) group , A779 group and control group respectively .Ang(1-7) group was significantly less than the control group . The difference has statistically significant (P<0.05) . (6) The cell count of Transwell chamber migration assay was 247,370 and 385 in Ang- (1-7) group , A779 group and control group respectively . Ang(1-7) group was significantly less than the control group . The difference has statistically significant (P<0.05).Conclusions: (1) Ang-(1-7) can promote cell apoptosis and inhibit the cell proliferation in hepatocellular carcinoma(HCC) of Huh-7 cells which are cultured in vitro .A779 can inhibit cell apoptosis and promote the cell proliferation in hepatocellular carcinoma(HCC) of Huh-7 cells which are cultured in vitro . (2 ) Ang-(1-7) can inhibit invasive and metastasis ability in hepatocellular carcinoma(HCC) of Huh-7 cells which are cultured in vitro through the G-protein coupled Mas. A779 is not obvious in hepatocellular carcinoma(HCC) of Huh-7 cells which are cultured in vitro through the G-protein coupled Mas.