Clinical And Experimental Study On The Treated Of Herpes Simplex Viral Keratitis From "Liver"

Purpuse: 1.Observe the clinical effect of treated from liver viral keratitis.2.Using BALB/c mice model,standard strains of infected animals HSV-1(F),normal mice abdominal cavity anesthesia,vaccine virus corneal scratch method,simple viral keratitis model is established,and model to investigate the liver treatment(lowering liver party)the curative effect and its mechanism of action.Methods: 1.Starting from the etiology and pathogenesis of corneal disease treatment of this disease are discussed,the research for the prospective,randomized,double-blind,controlled clinical trial simulation selection in our hospital in December 2012 to December 2014 viral keratitis patients,hospitalized with experimental income group 109 examples,fall off 21 cases,88 cases,using traditional Chinese medicine syndrome differentiation can be divided into liver by wind heat type and liver Yin deficiency type A total of 4 groups: type 2 A-liver wind heat type(A1,A2 in the treatment group);Type B-liver Yin deficiency,B2 treatment group(B1 control group).Control group to give more wast,respectively.there eye drops + 1 or placebo 2 Chinese medicine,Chinese traditional medicine placebo treatment group given the ganciclovir eye drops + traditional Chinese medicine(TCM)respectively.there one 1 or herbal medicinal broth 2,compare two groups of treatment effect,the time of symptoms and signs disappeared,and the adverse reactions.2.Preparation of lowering liver party,acyclovir drug-containing serum,in vitro antiviral experiments;BLAB/C mice,intraperitoneal injection of chloral hydrate anesthesia,surgical microscope with 5 blade tip on the back in the right eye cornea is character "#" Nick,with penetrating cornea cortex advisable,through a straw wil be 5 ?L containing or not containing 2 x 106 PFU HSV-I virus DMEM medium drops in the right eye cornea surface,HSK animal model is set up.Building after every day with Genesis-d eye camera observing eye infection situation in mice,and the corneal lesions;Respectively within 7 days after the building to be put to death and separation BLAB/C mice inguinal lymph nodes,extraction of single cell suspension,and T lymphocytes of isolation and purification of Ficoll density gradient centrifugation by flow cytometry instrument testing each antigen specific T cells of CD4+IFN-?+and CD4+IL-17+ percentage change;The heart blood is 0.5~0.6 m L,ELISA to detect cytokine IFN-?,the expression of IL-17.Results:1.Treatment group(A2,B2),blurred vision,photophobia,tears,the sand acerbity pain,ciliary hyperemia and cornea infiltrating edema disappeared time(A1,B1)faster than the control group,significant difference were observed in the comparison,the two groups(P < 0.05);Treatment group(A2,B2)(A1,B1)and control group cure rate and recurrence rate comparison,the differences were significant(P < 0.05).2.Lowering the drug-containing serum liver party had no significant effect on Vero cell proliferation,and less on Vero cell toxicity;Functions of liver treatment group compared with model group virus,Balb/c mice corneal lesions significantly narrowed down,reduce the infiltration point,the difference was statistically significant(P < 0.01),the functions of liver treatment group to 14 days,Balb/c mice basic cure corneal lesions,the average healing time 14.66 d;Each antigen specific CD4+ T cells in mouse lymph nodes CD4+IFN-?+,CD4+IL-17+ percentage(%),difference have statistical significance(P < 0.01);Functions of liver treatment group,ACV group listed T cell secretion of cytokines in mice(IFN-?,IL-17)expression compared with model group mice,the differences were statistically significant(P < 0.05).Conclusions: 1.From the liver to elucidate the clinical effect of viral keratitis,can significantly improve the cure rate and reduce the recurrence rate,worthy of clinical popularization and application.2.Traditional Chinese medicine(TCM)pathogens of HSK HSV-I have obvious inhibitory effect.By traditional Chinese medicine compound functions of liver curative effect observation of experimental HSK animals,medicated serum in vitro antiviral in vitro and in vitro cell based developing related experimental study such as antiviral,provided a objective basis for Chinese medicine treatment of viral keratitis.Antiviral mechanism of TCM and on the other hand may be linked to inactivated virus directly or induce interferon,reduce or block the virus m RNA translation;On the other hand to adjust the immune system,while the latter is more important.