Effects Of Over-expressing Smad7 In TGF-?/Smad Signalling Pathway On Keloid Fibroblasts

Objectives:To research the effects of over-expressing Smad7 gene on KFb by Observing the gene expressions of Smad7,collagen type,and collagen type ? and cell proliferation after over-expression vectors of Smad7 transfecting KFb.Methods:Fibroblasts were acquired from 10 male patient with keloids at department of Plastic Surgery,Second Affiliated Hospital,Kunming Medical University.After in vitro culture,KFb were divided into 3 groups:untransfected group(group A),pcDNA3.1(-)transfected group(group B),and pcDNA3.1(-)-smad7 transfected group(group C).The mRNA and protein expression levels of Smad7,collagen type ?,and collagen type ? were detected by real-time fluorescence quantitative PCR and Western blot at 48 hours after transfection.The cell proliferation ability was detected by MTT assay at 24 hours,48hours,72hours after transfection.Results:The relative expression levels of mRNA and protein of Smad7 in group C were significantly higher than those in group A and group B(P<0.01).The relative expression levels of mRNA and protein of collagen type ? and collagen type? in group C were significantly lower than those in group A and group B(P<0.01).The relative expression levels of mRNA of collagen type ? and collagen type ? in group B were significantly higher than those in group A(P<0.01);and the relative expression levels of proteins of Smad7,collagen type ?,and collagen type ? were significantly lower than those in group A(P<0.01).The cell proliferation ability in group C was significantly lower than that in group A and group B at each time point by MTT assay(P<0.05),but no difference was found between group A and group B(P>0.05).Conclusions:Gene expressions of collagen type ?,and collagen type ? and cell proliferation will be inhibited after KFb are transfected by over-expression vector of Smad7.