The Study Of TGF-?1 Induced Epithelial-mesenchymal Transition And The Wnt Signaling Pathway In Gastric Cancer Cells

Objective:Gastric cancer is currently one of the most common gastrointestinal malignancy,incidence is increasing in recent years.Even if the patient received surgical treatment and chemotherapy,the treatment effect was still not ideal.Gastric cancer metastasis is the most important characteristics of malignant tumor and the most of the major causes of death in cancer patients.Tumor metastasis is a multi-step process,at the beginning of the metastatic tumor cells in the process of(Epithelial-Mesenchymal Transition,EMT).At present,the study has found that transforming growth factors and Wnt/?-catenin signaling pathways in cancer play an important role in the process of EMT.Our purpose is to investigate the phenotypic changes of EMT and the mechanism of action of Wnt signaling pathway in the process of gastric cancer metastasis,and to provide a new theoretical basis for gastric cancer metastasis,and to provide experimental basis for targeted therapy of gastric cancer metastasis.Methods:After the cytokine TGF-?1 in different concentration(5/10/20/50ng/ml)was added to the complete medium and gastric cancer cells(MNK28)were co-cultured 72 hours,morphological changes were observed by using an optical microscope,cell phenotypic changes,cell invasion and metastasis ability were observed by CCK8 cell cycle assay,wound-healing assay and transwell chamber assay in induced cell linees compared with parental contrast cell lines and wnt signaling pathways of EMT markers(Wnt3a,?-catenin,CyclinDl)were detected bywestern blotting assay.Results:TGF-?lin different concentration of(5/10/20/50ng/ml)induced gastric cancer cells EMT in vitro and changed gastric cancer cells morphology.So gastric cancer cells adhesion weakened,cells viability increased,proliferation ability and invasion ability ofcells increased.the expression of Wnt signaling pathways of EMT markers(Wnt3a??-catenin?CyclinD1)up-regulated by western blotting assay.However,the induced effects were not correlated with the concentration of TGF?1 and the best induction concentration was 10ng/ml.Conclusions:1.TGF-?1 in different concentration can induce gastric cancer cells EMT.2.TGF-?1 can promote gastric cancer cells EMT by effectively activating Wnt3a signaling pathways.3.The induced effects were not correlated with the concentration of TGF?1.