| Objectives:Moderate brain injury in mice caused by hydraulic shock,after injury with different forms of moderate intensity aerobic exercise rehabilitation training,observing the effects of exercise for brain injury in mice after the motor function,cognitive function and mouse brain tissue injury;and to explore whether aerobic exercise intervention after brain injury,injury in chronic stage the peripheral stress hormone cortisol,and proinflammatory cytokines IL-6,IL-12 levels.So as to provide reference basis for the rehabilitation model of brain injury.Methods:The lateral hydraulic shock mice in motor and sensory areas caused moderate brain injury,trauma after 6 days of exercise rehabilitation training in two stages for a period of 28 days.The training modes were A:using the special mouse treadmill to carry out the passive variable interval training;B:using the rotary cage to carry on the independent continuous training.The mice were examined with cylinder test,grid walk test,tail suspension test and field test to detect the motor function and cognitive function.Select the change of multiple time points for cylinder test,grid of mice with motor function on mice after traumatic brain injury in walking test after traumatic brain injury and effects of exercise on function of mice after traumatic brain injury and two kinds of rehabilitation training mode;and after traumatic brain injury selected multiple time points in mice tail suspension test,pile field test and Y maze test to explore the mice after trauma,anxiety and depression,cognitive function changes,and the effects of two kinds of rehabilitation training after traumatic brain injury in mice model of anxiety and depression,cognitive function.At the end of the test mice behavior of orbital blood and extract serum,serum of mice cortical ketone,IL-6,IL-12 were detected using ELISA kit,serological index and the behavior indexes for correlation analysis.Blood was taken immediately after the mice were perfused and brains,the brain is fixed,remove the mouse cortical lesions of coronary Chetty,gradient alcohol dehydration,paraffin embedded by paraffin section.Sections were dewaxed after routine hematoxylin eosin staining and Nissl staining.Under microscope,the neurons of cortex were observed.And the serial sections were used to calculate the volume of brain damage in the injured cortex.Results:Posttraumatic treadmill rehabilitation training group mice weight,cage mice weight training group,and trauma group were significantly higher than those of trauma;after operation,3 groups of mice induced by trauma in motor function showed decreased significantly,the specific performance of the cylinder test in the ipsilateral forelimb decline,forelimb use asymmetry scores increased,in the grid walk test midleg error ratio increased;simultaneously depression,the specific performance of the tail suspension test in stationary time;and the emergence of anxiety,the specific performance of mice in the field test in the middle of the 1/4 area of the total movement distance distance ratio decrease;decline in space cognitive ability the specific performance in the Y maze,automatic rotary measure automatic rotarypercentage decline in the new exploration resolution coefficient test drop arm.After two stages of rehabilitation training,the training level of the motor function and cognitive ability of the treadmill training group and the cage training group were better than that of the trauma group.The cerebral cortex of the mice was reduced to the size of the cage rehabilitation training group.The number of neurons in the cerebral cortex injury group,treadmill rehabilitation training group,rehabilitation training group,cage control group,trauma group and cortical neurons arranged in disorder,pyknosis serious cavitation,the control group of cortical neurons arranged in neat,clear nucleolus,between the two groups of rehabilitation training between the two groups of neurons form.The levels of serum corticosterone,IL-6 and IL-12 in the trauma group were higher than those in the rehabilitation training group and the treadmill training group.There was no statistical difference between the two groups.Conclusions:1.The effect of hydraulic shock on the motor function and cognitive function of mice.The blood-brain barrier damage caused by traumatic brain injury significantly increased the levels of IL-6 and IL-12 in serum.Traumatic brain injury caused a significant increase in the level of stress in mice,peripheral blood pressure hormone corticosterone levels increased significantly.2.After injury rehabilitation training can reduce the sensitivity of mice to stress and improve the level of depression and anxiety.To improve the rehabilitation training of rehabilitation training on motor function and cognitive function in mice may be the blood-brain barrier by repairing damaged mice,alleviate the inflammatory damage to the integrity of the neurons decreased the damage volume of brain tissue to maintain nerve function can be achieved.3.The comparison of two kinds of training mode of training results,posttraumatic treadmill rehabilitation training on anxiety,reduced the level of IL-12 is better than that of self rotating cage rehabilitation training after trauma;independent cage rehabilitation training on depression recovery,ipsilateral forelimb motor ability than the treadmill rehabilitation training and rehabilitation training in the self rotating cage reduce the volume loss of the injured side effect is better;but there was no difference in serum corticosterone and IL-6 two kinds of rehabilitation training mode. |
