The Preparation Of Millet (Setaria Italica) Bran Ethanol Extractive And Study On The Antioxidant Activity

The millet bran is the byproduct of millet peel,seed coat.lt has high nutritional value and many other advantages in medical field,such as improving human immunity,the ability of the anti-aging and antioxidation.This paper set the millet bran which from Jiaxian in northern Shaanxi as the research goal.the conventional physical and chemical indicators are measured by the standard reference methods.Meanwhile,the ethanol extractive of millet bran extraction process were optimized by the single factor experiment and response surface methods.Through the determination of DPPH free radical clearance,Fe3+ resilience(FRAP),Fe2+complex force,and the total reducing power in vitro antioxidant activity indicators,then combination of the Single cell gel electrophoresis test(SCGE),the antioxidant activity of the extractive was evaluated,in order to obtain the application value of antioxidant activity,provide the basis for the development and utilization of millet bran active ingredients.The main results of this paper is as follows:1.The main physical and chemical indexes of millet bran:the content of crude fat was 25.71%,the moisture content was 10.17%,the ash content was 8.98%,the total value of protein was 13.11%,reducing sugar contained was 7.45%,then the crude fiber was 33.11%,and the percentage of saturated fatty acids was 9.66%which was determined by gas chromatography(GC)method,the results show that the percentage of unsaturated fatty acids was 90.34%of fatty acid content;2.Using single factor experiment combined with the response surface analysis,to comprehensively investigate the optimal ethanol extraction process conditions of millet bran:the ratio of material to liquid was 1:16,the ultrasonic time was 34 min,the ultrasonic power was 230 w,the volume fraction of ethanol was 90%,with this condition,the extraction yield of millet bran which was extracted by ethanol was 10.73%;3.The ethanol extractive of millet bran was extracted by the optimized process conditions,with different vitro and in vivo antioxidant activity test to evaluate its antioxidant activity,the alue concentration of IC50 of millet bran extraction to remove DPPH free radical was 1.46 mg/mL,and the correlation coefficient with total phenolic acid content(R2=0.9255);the Fe3+reduction ability(FRAP)was 97.79 mg Trolox/kg,with total phenolic acid content correlation coefficient R2 = 0.9179;In complex power of Fe2+ was 36.36±3.75 mg EDTA-Na2/kg,with total phenolic acids content of correlation coefficient R2 = 0.4679;The total reducing power was 1.12,and total phenolic acids content of correlation coefficient R2 = 0.783.The ethanol extraction of millet bran could inhibit the oxidative damage of DNA content in lymphocytes of mice,and was positively correlated with the concentration of the extractive,the relationship between low dose rate of extract and high doses of the extracts from the tail to 26.0%and 19.0%respectively,indicating that the ethanol extractive of millet bran has certain protective effect for the DNA oxidative damage induced by H2O2.