| Millet, is Also known as the corn. Millet bran is the by-product in the process of millet in the processing, which is composed of the chaff, seed coat, aleurone layer and germ. The yield of millet in our country is high, which reached 90% of the world’s corn production. the nutritional value of millet polished bran is rich, The protein content of which accounted for 13%～18%. At present millet bran is regarded as livestock feed in most countries, it might lead to nutrition in millet bran has not been fully utilized. Scientific research studies of rice bran protein and peptide is more than the millet bran protein and polypeptide. Development of health care products about millet bran protein and polypeptide has a good prospect, which can not only make full use of millet bran nutritional ingredients and reduce the pollution to the environment. In this study the defatted millet bran was a main raw material which was aimed to investigate the antitumor activity of millet bran poly-peptides (MBPE) and their inhibitory effect on chemically induced liver injury, providing the scientific basis for functional millet bran peptide product research and development. The research contents of this paper are as follow:1. The extraction of millet bran albumin:To dip the defatted millet bran in the distilled water according to material-liquid ratio 1:10, then millet bran needed to be hydrolyzed for 3h under the condition of 55℃, pH4.5 with composite tannin enzyme, at last millet bran albumin has been extracted for 6h at the condition of pH 7.0,55℃,the extraction ratio of albumin can reach 48.96%.2. The preparation technology of millet bran polypeptides (MBPE): Millet bran albumin was hydrolyzed with a variety of acidic, neutral and alkaline protease to extract millet bran polypeptides, on the basis of polypeptides extraction yield, After the screening, the hydrolysis effect of neutral protease S, bromelain, Acid protease P1 is better. The best condition of the above three kinds of protease peptide yield is as follows:90.27%, 87.51% and 79.72%.3. MBPE in vitro antitumor test and immunity test:MTT assay was applied to detect the in vitro antitumor effect of MBPE prepared with above three kinds of enzyme, the best effect of MBPE was selected and used as raw materials for the animal testing. By measuring the spleen index and thymus index, the number of white blood cells and value of half hemolysis to measure MBPE influence on immune function in mice. It was found that MBPE promote the immune organ development, improve the body immunity.4. Inhibitory effect of MBPE on liver injury and precancerous lesions: Healthy kunming mice were treated as the research object, feeding amidopyrine-sodium nitrite (AP-NaNO2) to establish the model of precancerous lesions mice, The experimental mice were feed MBPE which had best effect in vitro antitumor with high and low dose respectively. After 16 weeks, the content of liver malondialdehyde (MDA), triglycerides (TG) and the serum levels of alanine aminotransferase (ALT), aspertate aminotransferase (AST) were determined, the liver surface change and liver tissue pathological slices were observed. It turned out that MBPE had significantly inhibitory effects on liver injury and precancerous lesions.5. Inhibitory effect of MBPE in different molecular weight on liver injury and precancerous lesions:Ultrafiltration was utilized to separate MBPE which has best effect in vitro antitumor, experimental mice were feed MBPE with Large and small molecular weight. The content of liver malondialdehyde (MDA), triglycerides (TG) and the serum levels of alanine aminotransferase (ALT), y-GT were determined, and liver tissue pathological slices were observed. The experimental results showed that small molecule MBPE had a significant inhibitory effect on liver injury and precancerous lesions. |
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