| Background:Prostate cancer is a common malignant tumor of the male genitourinary system.As the population ages and changes in lifestyle,there is an increasing trend in incidence of prostate cancer,which have been the second place in Asia including China.The occurrence and development of prostate cancer is close related to androgen receptor(AR).With the prostate cancer cells malignant degree increasing,the AR expression reduces.The expression of AR in early prostate cancer is higher than the late prostate cancer,and the high and moderate differentiation prostate cancer is higher than low differentiation prostate cancer.Bombardelli L et al.found that epithelial tumor cells secrete immunoglobulin G(IgG)which causes immune escape and promotes tumor cell growth by inhibiting Antibody-dependent Cell-mediated Cytotoxicity.In the early study,we found the IgG expressed in prostate cancer,and with the increase of tumor malignant degree,IgG expression increases,implicated that the IgG may play an important role in the process of the incidence of prostate cancer.Consequently,is there a correlation between AR and IgG of prostate cancer and what the mechanism of its action?So far,there is no related research at home and abroad.We proposed by intervening the expression of AR in prostate cancer cells,then detecting IgG expression and the change of the prostate cancer cells biological characteristics including proliferation and migration.Hereby,we intended to make clear the relationship between AR and IgG of prostate cancer.Objective:To investigate the effect of modulating AR on IgG synthesized by prostate cancer cells and the proliferation and migration of prostate cancer cells.Methods:(1)The expression of AR protein and IgG synthesized by LNCap,androgen-dependent prostate cancer cell and PC-3,castration resistant prostate cancer cell were respectively detected by Westem blot.(2)Build cell model:PC-3 cells were transfected via lipofectamine by the pCDNA3.1+-AR overexpressing AR gene.LNCap cells were transfected via lipofectamine by siRNA.(3)Detect related parameter:The expressions of AR protein and IgG synthesized'by cancer cells were detected by Westem blot.The expression of IgG mRNA was detected by Q-PCR.Cancer cell proliferation and migration were respectively detected by MTT assay and wound healing assay.(4)Statistical analysis:SPSS 2.0 statistical software was used for data processing.All data in the present studies were reported as mean ± SE.One-way ANOVA(Bonferroni method)was used to test the statistical differences of the PC-3,PC-3-V,PC-3-AR cells and LNCap,LNCap-siCon,LNCap-siAR cells multiple sets of IgG mRNA and protein expression;P<0.05 was considered statistically significant.Results:(1)Compared with the PC-3 cell,LNCap cell had a higher expression of AR protein and a lower expression of IgG(P<0.05).(2)After the PC-3 cells were transfected via lipofectamine by the pCDNA3.1+-AR overexpressing AR gene,the PC-3-AR cells represented a higher expression of AR and lower expression of IgG(P<0.01),with a reduction in proliferation and migration,compared with the PC-3 cells.The expression of IgG mRNA of the PC-3-AR cells was significantly lower than the PC-3 cells and the PC-3-V cells(P<0.01).After the LNCap cells were transfected via lipofectamine by siRNA,the LNCap-siAR cells represented a lower expression of AR and higher expression of IgG(P<0.01),with an increase in proliferation and migration,compared with the LNCap cells.The expression of IgG mRNA of the LNCap-siAR cells was significantly higher than the LNCap cells and the LNCap-siCon cells(P<0.01).Conclusion:Regulating the exression of AR made an impact on the expression of IgG wihch induced the change of the proliferation and migration of the prostate cancer cell.By upregulating the expression of AR of prostate cancer cells,the expression of IgG downregulated and the tumor malignant degree reduced.By downregulating the expression of AR of prostate cancer cells,the expression of IgG uregulated and the tumor malignant degree increased. |
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