Activity Strengths Of Cortical Glutamatergic And GAB Aergic Neurons Are Associated With Transgenerational Inheritance Of Learning Ability

Objective: To study the coordinated plasticity at glutamatergic and GABAergic neurons under the transgenerational regulation of learning ability.Methods: 1.Model building: select C57BL-6-gfp mice(mice for short)that was born 15 days giving butyl acetate as the source of the smell stimulation of the olfactory stimuli at the same time giving the right beard 5Hz..Each of the mice was trained twenty seconds in each time,five times per day with two hours of intervals for ten days.During the training,each mouse was placed in a home-made cage.Cares were taken including no stressful experiment condition and circadian disturbance to the mice that showed normal whisking and symmetric whiskers.2.Monitoring: Whisker motion tracks were monitored by a digital video camera(240 Hz)and were quantified in retraction time and whisking frequency.The responses of mouse whiskers to the odor-test(butyl acetate,20 sec)were measured before the training and at the end of each training day to quantify the onset time and levels of conditioned reflex(CR).CR-formation was defined to meet the following criteria.The patterns of odorant-induced whisker motion were similar to those of WS-induced whisker motion.As this type of whisker motion induced by odorant was originally induced by WS,the odor signal initiated a recall of whisker signal and then led to whisker motion.Learning efficiency was measured based on the time at the fully establishment of odorant-induced whisker motion.According to our data from all of the mice in the expression of odorant-induced whisker motion,we defined learning ability as either high or low efficiency in following.If their odorant-induced whisker motion reached to the plateau level at training day six,they were defined as the mice with high learning efficiency(HLE).If odorant-induced whisker motion reached to the plateau level at training day 10 or after,they were named as the mice with low learning efficiency(LLE).In our studies,HLE male and female mice,HLE female mice and LLE male mice as well as HLE male mice and LLE female mice were cross-mated,such that their filials in the first generation(F1)were placed into three groups,respectively.These F1 mice were trained by pairing whisker and odor stimulations in order to examine their learning efficiency versus training days.3.Electrophysiological experiments: Based on these dynamic curves,the brain slices from these F1 mice were made at training day six,and their barrel cortical glutamatergic and GABAergic neurons were recorded to analyze the efficacy of synaptic transmission and the ability to convert excitatory inputs into digital spikes.Results: 1.Behavior results: The efficiency of associative learning appears a sequence of high-to-low strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice.This result indicates that the parents with high learning ability appear able to generate the offsrpins with high learning ability,or vice versa,especially female in dominance.2.Electrophysiological experiments: The distance of the action potential of the Glutamate neuron in the barrel cortex appears a sequence of high-to-low strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice.The issue number of potential action potential volume per unit time increased.The s EPSC interval of events of the Glutamate neuron in the barrel cortex appears a sequence of low-to-high strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice,and the amplitude was converse;the s IPSC interval of events of the neuron in the barrel cortex appears a sequence of high-to-low strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice,and the amplitude was converse.The distance of the action potential of the GABA neuron in the barrel cortex appears a sequence of low-to-high strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice.The issue number of potential action potential volume per unit time reduced.The s EPSC interval of events of the GABA neuron in the barrel cortex appears a sequence of low-to-high strength on HLE in F1 mice that are offsprings of HLE male and female mice,middle learning efficiency(MLE)in F1 mice from HLE female and LLE male mice as well as LLE in F1 mice from HLE male and LLE female mice,and the amplitude was converse.Conclusions: Activity strengths of cortical glutamatergic and GABAergic neurons are associated with transgenerational inheritance of learning ability...