Coordinated Plasticity Of Excitatory And Inhibitory Neurons Is Associated With Learning Transgenerational

Objective: Study the formation of excitability neurons and inhibitory neurons in learning transgeneration under associative learning and memory model in C57BL‐6‐gfp mice.Methods: 1. Model building: select C57BL‐6‐gfp mice(mice for short) that was born 15 days giving butyl acetate as the source of the smell stimulation of the olfactory stimuli at the same time giving the right beard 5Hz for 1 minute,two hours at a time, 5 times a day, keep training for 10 days.2. Monitoring: After training, give olfactory stimuli only to recording mouse whiskers swing by take videos every day, motion tracking whiskers swing path by using Image J software. Based on swing tracks to divide the mice to high effective learning groups and low efficient learning groups.3.Learning transgenerational model: high effective learning mice paired with high effective learning mice so was the low efficient group, monitoring filial generation mice in behavior experiment to see whether have differences in learning ability.4. Electrophysiological experiments: to training the offspring mice for 5 days on each of the two groups conducted electrophysiological experiments. Focus on changes of intrinsic and synaptic transmission efficiency of glutamate neurons and GABA neurons in barrel cortex.Results: 1. Behavior results:1) High efficiency filial generation and low efficiency filial generation group in training 4, 5, 6, 7th days have significant difference in learning ability, correspond with its parent.2) Electrophysiological experiments:A. Current clamp experiments:1). Glutamate neurons: Compared with low efficiency filial generation group, the distance of the action potential of the neuron in the barrel cortex the high efficiency filial generation group was shortened, the absolute refractory period was shortened even more significant, the issue number of potential action potential volume per unit time increased.2). GABA neurons: compared with low efficiency filial generation group, the distance of the action potential of the neuron in the barrel cortex the high efficiency filial generation group was increased, the absolute refractory period was extended even more significant, the issue number of potential action potential volume per unit time reduced.B). voltage‐clamp test results:1).glutamate neurons: compared with low efficiency filial generation group, the s EPSC interval of events of the neuron in the barrel cortex the high efficiency filial generation group was shortened, and the amplitude was increased; the s IPSC(spontaneous inhibitory postsynaptic currents) interval of events was increased, the amplitude was decreased more significantly.2) GABA neurons: compared with low efficiency filial generation group, the s EPSC interval of events of the neuron in the barrel cortex the high efficiency filial generation group was shortened, and the amplitude was increased.Conclusions: Learning ability could across generations; this transgenerational associative with coordinated plasticity of neurons.