Effect Of Small Interfering Rna Target Nfia On Invasion And Migration Ability Of Human Glioma Cells

Objective The aims of the present study are to evaluate the role of the NFIA in glioma development and progression and to clarify cytology mechanisms of its high expression with distal metastasis.Methods:We also determine the regulatory mechanisms between NFIA and ezrin,and its underlying molecular mechanism in regulating glioma metastasis.Methods Small interfering RNA(si RNA)targeting NFIA was synthesized and transfected into glioma cell line U251 cells,and the expression of NFIA were detected in the transfected glioma cells by real-time PCR and western blotting assays.Transwell assay and wound healing assay were used to determine the invasion and migration of the cells,respectively.Results The expression was significantly up-regulated in glioma tissues as compared with normal brain tissues.The m RNA and protein expression levels of NFIA in RNAi group(si RNA2 and si RNA3)were significantly lower than those in the negative control group and blank control group(P<0.05);Wound healing assay indicated that the scrape wound recovered significantly lower in the NFIA RNAi group(57.9±6.9)?m than that in the negative control group(125.4±10.7)?m and blank control group(111.5±5.9)?m(P<0.05);Transwell assay showed that the invasiveness capability of cells in NFIA RNAi group(33.3±3.2)was significantly decreased as compared with that in the negative control group(80.3±4.5)and blank control group(87.7±5.0)(P<0.05).The expression of Ezrin in NFIA RNAi groupwas significantly decreased as compared with that in the negative control group and blank control group.Conclusions NFIA in the glioma tissues are significantly increased as compared with those in the normal brain tissues.Down-regulation of NFIA inhibited glioma cell invasion and migration ability.