| Objective：To investigate relationship between the RNAi silencing of ERK1/2and expression of Ezrin gene in human gastric cancer in vitro.Methods：The expression of Ezrin in80specimens of gastric carcinoma,80specimens of normal gastric tissues were detected by immunohistochemistry method.Conventional culture of gastric cancer cells SGC7901, two siRNAs targeting ERK1and ERK2respectively were designed and synthesized according to its mRNA, andtheir corresponding expression vectors were constructed and transfected intoSGC7901cell with LipofectamineTM2000. The inhibition of ERK1and ERK2expression, together with the expression of Ezrin were analyzed by RT-PCR, theprotein level of those genes were detected by western blot analysis. Transwell methodwas applied to measure cell invasion and migration.Results：In normal gastric tissues and gastric carcinoma, the percentage of Ezrinpositive expression was23.75%and88.75%, respectively. The difference has astatiftical significance (P<0.05). Compared with the controls, the groups of ERK1RNAi and ERK2RNAi effectively inhibited the expression of ERK1and ERK2, withinhibitory rate of60.37%and61.8%, among the two groups, the expression of Ezrinwere down-regulated by62.62%and51.37%respectively with significientlydecreased cell invasion and migration (P<0.05).Conclusion：Compared with normal gastric tissues, Ezrin was over-expression ingastric carcinoma tissues. The expression of ERK1and ERK2in SGC7901cellscould be supressed by vetor-based RNA interference efficiently. The knockdown ofERK1/2caused changes in Ezrin gene expression related with cell invasion andmigration, finialy resulted in decreased cell invasion and migration. |
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