MiR-182 Et Al Expression In Prostate Cancer Samples And The Effect Of Rs2016347 Ploymorphism On MiR-3175 Binding

Part ? miR-182 et al expression in prostate cancer samplesObjective To study the relationship between the microRNA co-screening and the diagnosis of prostate cancer in order to provide a theoretical foundation for exploring the possible biological mechanism of prostate cancer development,and a more effective guidance to detect the potential biomolecule markers for prostate cancer screening and to optimize implementing clinical diagnosis.Methods RT-qPCR was applied to measure the expression of five miRNAs screened in the prostate cancer tissues and the adjacent tissues.The five miRNAs were then analyzed by SPSS software.Results To measure the expression of the five miRNAs found in the previous data through RT-qPCR analysis,the expression of miR-182-3p,miR-182-5p,miR-200 c were found higher in prostate cancer tissues than that in the adjacent tissues,the miR-182-5p up-regulated apparently(p=0.0018),miR-200c(p=0.0031),and miR-182-3p(p=0.005),whereas the expression of miR-211-3p and miR-221-5p was lower in prostate cancer tissues than that inthe adjacent tissues of prostate cancer,miR-221-3p down-regulated significantly(p=0.0088),then followed by miR-221-5p(p=0.0319).The area under the AUC curve obtained by the ROC combined diagnosis of the five miRNAs was 1.Conclusion Among the Han nationality,the expressions of miR-182-3p,miR-182-5p,and miR-200 c were found higher in prostate cancer tissues than that in the adjacent tissues of the prostate cancer.The expression of miR-182-5p was up-regulated most,while that of miR-221-3pand miR-221-5p was down-regulated,miR-221-3p down-regulated most.These five miRNAs altogether tend to be the markers of prostate cancer screening.Part ? The effect of rs2016347 polymorphism on miR-3175 bindingObjective To study the polymorphism rs2016347 in IGF1 R 3?untranslated region(UTR)that is associated with prostate cancer(PCa)and affected disease risk by altering miRNA binding.Methods The targeted miRNA was predicted through miRbase,and the thermodynamic model method was applied to calculate the binding energy.Furthermore,dual luciferase reporter assay was used to verify whether binding affinity of miRNA was influenced through altering allele of rs2016347 in HEK293 T cell in vitro.Results Prediction of miRbase demonstrated that binding region of miR-3175 and IGF1 R was located in the polymorphism rs2016347.Calculation results of thermodynamic model method indicated that T allele of rs2016347 would generate a stronger binding of hsa-miR3175 on IGF1 R 3'UTR compared with the G allele.Dual luciferase reporter assay indicated miR-3175 binding to IGF1 R 3' UTR contained G allele or T allele of rs2016347,and showed a stronger binding with T allele.The binding of miR-3175 and IGF1 R gene was playing a role in stabilizing the IGF1 R gene expression.Conclusion Polymorphism rs2016347 with T allele showed a greater risk in the progression of PCa.