| Part1 Expression of caspase-1 and its downstream inflammatory factor IL-1? in rat model of incisional painObjective:To investigate the changes of caspase-1 and downstream inflammatory factor IL-1 beta expression in rat model of incisional painMethods:Thirty-six 240±10g adult male Sprague Dawley?SD?rats were randomly divided into two groups?n=18?: incisional pain group?group I?and control group?group C?.group I the model of incisional pain was adopted as described by Brennan et al,group C didn't do any processing.To evaluate the behaioral changes,paw mechanical withd-rawal threshold?PMWT?Were measured at 24 h before incision?T0?and at 2 h?T1?,6h?T2?,24 h?T3?,48 h?T4?point after incision operation.The animals were sacrificed after PMWT was measured,Each group was randomly selected 6 rats,the L4-L6 segments of spinal cord Was dissected out,The expression of Caspase-1?P20?was detectedby immunohistochemistry and the expression of caspase-1mRNA was detected by RT-PCR.The expression of IL-1 beta protein was detected by enzyme linked immunosorbent assay?ELISA?.Results:1.The changes of MWT in the process of postoperative painThere were nosignificant differences in the value of MWT between group I and group C hind-paw of the rats on the value of MWT before incision?P >0.05?;Compared with group C,PMWT was lower at T1-T4 point postoperation in the group I?P < 0.05?;Compared with group T0,PMWT was lower at T1-T4 point postoperation in the group I?P > 0.05?.2.Expression of spinal Caspase-1 mRNA and Caspase-1 protein?IL-1 beta protein in rat models of postoperative pain.Compared with group C,The expression of spinal Caspase-1 mRNA and Caspase-1 protein?IL-1 beta protein in rat models of postoperative pain in group I significantly increased?P < 0.05?.Part ? Effects of intrathecal Caspase-1 inhibitor on rat model of incisional painObjective: To investigate the effects of intrathecal Ac-YVAD-CMK?caspase-1 inhibitor?on rat model of incisional painMethods:Eighteen 240±10g adult male Sprague-Dawley rats in which the catheter was successfully placed in the intrathecal were randomly divided into 3groups?n=6 each?:incisional pain model group?group I?,vehicle group?group IR?,inhibitor group?group IJ?.Normal saline?20 ?l?or vehicle?20 nmol?Ac-YVAD-CMK?20 nmol?was intrathecally administrated prior to incision in the rats,To evaluate the behaioral changes,the PMWT value of the anterior?T0?and posterior?T1?catheter of the rat intrathecal?2?T2?,6?T3?,24?T4?and 48 h?T5?point after postoperation were measured.The animals were sacrificed after PMWT was measured and L4-L6 segments of spinal cord Was dissected out.The expression of IL-1 beta protein in spinal cord was detected by enzyme linked immunosorbent assay?ELISA?.Results1.The changes of MWT in the process of postoperative painThere was no significant difference in PMWT value between the three groups before and after intrathecal administration?P > 0.05?;Compared with group I,PMWT was higher at point T2-T5 point postoperation in the group IJ?P< 0.05?;Compared with group I,PMWT at point T1-T4 point postoperation was no significant difference in the group IR?P > 0.05?.2.Expression of spinal IL-1 beta Protein in rat models of postoperative painCompared with group I,the expression of spinal IL-1 beta protein in rat in group IJ significantly increased?P < 0.05?;Compared with group I,the expression of spinal IL-1 beta protein in rat in group IR was no significant difference?P > 0.05?Conclusions : Caspase-1 was activated in rat model of acute incisional pain,increasing the expression of inflammatory factor IL-1 beta;Caspase-1activation in spinal cord may be involved in the formation of incisional pain through the development of the catalytic factor IL-1 beta... |
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