Studies Of Serum Fingerprint And The Main Components Serum Pharmacokinetics On Drug-couple Psoralea Corylifolial-myristica Fragrants Hout

Objective Herb-pairs of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout is widely used in clinic pracise.Two drugs combined with warm the kidney and the spleen and antidarrhea with astringent.In this study,We made Drug-couple Psoralea corylifolia L-Myristica fragrants Hout as a model drug to establish chromatographic fingerprint of components of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout absorbed into blood by UPLC.The aim is to explore the basis of pharmacodynamic substances.Using a rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry(UPLC-Q-TOF/MS)method to analysis constituents rapidly of before and after Drug-couple Psoralea corylifolia L-Myristica fragrants Hout after oral administration in rats.To conduct multiple-reaction monitoring(MRM)quantitative analysis with high performance liquid chromatography coupled with mass spectrometry method to investigate the pharmacokinetics process of the main components of before and after Drug-couple Psoralea corylifolia L-Myristica fragrants Hout after oral administration.Method1.20 SD rats were administered 10 different batches of Drug-couple Psoralea cory Lifolia L-Myristica fragrants Hout respectively.The UPLC profiles of serum samples collected after the treatment of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout,blank serum samples and methanol extract were compared,and the transitional constituents to blood and their metabolites were analyzed.2.We established a rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry(UPLC-Q-TOF/MS)method to analysis constituents rapidly of before and after Drug-couple Psoralea corylifolia L-Myristica fragrants Hout after oral administration in rats,and combined with Peakview software analysis. 3.36 rats were randomly divided into three group(A、B、C)and were orally psoralen extract,psoralen nutmeg extract,nutmeg extract respectively,the blood samples were collected at different time points.To conduct multiple-reaction monitoring(MRM)quantitative analysis with high performance liquid chromatography coupled with mass spectrometry method,establish the quantification method of psoralen,isopsoralen,bakuchiol,dehydrodiisoeugenol in blood sample under positive iron mode chloramghenicol as internal standard,investigate the pharmacokinetics process of the main components of before and after Drug-couple Psoralea corylifolia L-Myristica fragrants Hout after oral administration.Results1.To established 10 different batches serum fingerprint of Drug-couple Psoralea cory Lifolia L-Myristica fragrants Hout,13 common peaks were i-dentified,the similarities were over 0.90.The 10 peaks from the in vitro test prototype components in the product,3 peaks are metabolites.2.The chemical constituents of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout in vivo and in vitro results showed that compared with in vitro Psoralea corylifolia L,there are 17 prototype into the blood.Compared with in vitro Drug-couple Psoralea corylifolia L-Myristica fragrants Hout,28 prototype components into the blood.Compared with Myristica fragrants Hout,6 prototype components into the blood.3.To conduct multiple-reaction monitoring(MRM)quantitative analysis with high performance liquid chromatography coupled with mass spectrometry method,in the plasma samples,the linear relationship were good over the concentration rage of0.098125 to 39.25 for psoralen,0.08437 to 33.75 for isopsoralen,0.046875 to 18.75 for bakuchiol,0.11 to 2.2 for dehydrodiisoeugenol.The results of precision,stablity and extraction recovery tests showed that the determination method of plasma concentration for such compositions was stable and reliable.The four indicators are absorbed quickly,psoralen and isopsoralen have a double peak phenomenon.Tmax is significantly shortened after the match,Cmax and AUC0-t have varying degrees of growth.Conclusion The serum fingerprint of Drug-couple Psoralea corylifo Lia L-Myristica fragrants Hout is established.It reflects the oral administration absorption into the blood and provide some data on pharamacodynamic basis study in vivo for Drug-couple Psoralea corylifolia L-Myristica fragrants Hout.The UPLC-Q-TOF/MS method was used to make qualitative analysis on the chemical constituents of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout in vivo and in vitro and provide some data on pharamacodynamic basis study in vivo for Drug-couple Psoralea corylifolia L-Myristica fragrants Hout.The effect of compatibility on the kinetic parameters of the main components of Drug-couple Psoralea corylifolia L-Myristica fragrants Hout was studied by UPLC-MS/MS.According to the experimental results,the combined application of psoralen and nutmeg can significantly impact pharmacokinetic process,increase the blood drug concentration distribution and accelerate the metabolic and elimination in vivo.