Spleen Deficient Certificate In Xinjiang Uygur And Han Nationality With Psoriasis Vulgaris In IL23/Th17 Pathway Genetic Susceptibility Research

Objective: psoriasis is a chronic recurrent disease,is considering immune most related with heredity.Psoriasis is divided again,with psoriasis vulgaris,type pustular psoriasis erythrodermic psoriasis,joint type of psoriasis,the disease preval ence is higher in the world,and in the increasing trend year by year.We found the xinjiang region of spleen deficient type accounted for nearly half of psoriatic patients,this subject based o n the difference of xinjiang uygur and han nationality,on the basis of the resources of the xinjiang minority nationality,search and in the process of identifying the onset of ha n Chinese and uighurs in IL23 / Th17 signaling pathway gene epistatic effect model.Methods: Collected 3 cases of typical Uighur wet spleen deficient with psoriasis vulgaris patients blood samples,3 cases of healthy uighurs blood specimens,the blood samples by extraction,quantitative and quality inspection process,By Agilent technologies are centrifugal,synthesis,fluorescent tags,The Feature Extraction(version10.7.1.1,Agilent Technologies)software deal with original image to extract the raw data.After standardization of data filtering,in each group of samples used to compare a group of at least 100% of the probe is marked as Detected left for subsequent analysis.Use of p values of T test and multiple changes in the value differences between genetic screening,screening standard is raised or lowered multiples change value > = 2.0 and p < = 0.05.Then,to GO and KEGG enrichment anal ysis of genetic variations,to determine differences mainly affect the biological function of genes or pathway.Finally,the genetic variations of unsupervised clustering hierarchy,use the form of heat maps show differences in gene expression patterns between different samples.Finally through the comparison,the difference between screen related genes.Results: 1.Through the analysis of the differences in total,the test screening out 227 genes,which raised 120 genes,cut 107 genes;2.Leucocyte antigen in the signal path and HLA-A transcription error control of signaling pathways in cancer MPO expression is higher.Conclusion: Wet fill the Uighur patients with spleen deficient certificate in the presence of susceptibility genes.