Cardioprotective Effect Of Rutin On Myocardial Ischemia-reperfusion Injury In Rats

Objective To investigate the m echanism of rutin underlying its be neficial effect against myocardial ischemia reperfusion injury in rats.Method Male Sprague–Dawley rats were randomly divided into five groups: Sham,MI/R,RH(80 mg/kg),RM(40 mg/kg)and RL(20mg/kg)groups(n=10),establishthe myocardial ischemia-reperfusion injury m odel.The treatm ent groups were administered orally 30 min before myocardial ischemia.Echocardiography evaluation of cardiac function after myocardial ischemia/reperfusion injury were examined in the present study.Hemodynamic parameters including heart rate(HR)?left ventricular end-diastolic pressure(LVEDP),left ventricular systolic pressure(LVSP),and rate of contractility and relaxation L Vd P/dtmax and LVd P/dtmin were also m easured.The infarcted area of m yocardial infarction wa s determined Detection of myocardial enzyme production(CK-MB,LDH,and AS T)and oxidation index activity(ROS ?SOD?reduced GSH?MDA)in serum and tissue by enzym e-labeled instrument.Observation of cell morphology by hem atoxylin-eosin staining m ethod.The expression of Prx? and apopt osis related proteins(ie.Bcl-2,Bax,active-Caspase-3 and 9)were detected by Western blot..Results Compared with the sham group,the EF% and FS% of the model group were significantly decreased,LVEDP was significantly increased,LVSP and LVd P/dtmax were significantly decreased,with significant difference between two groups(P<0.01);myocardial enzymes were significantly increased(P<0.01);the production of ROS and MDA was significantly increased,the activity of SOD and the production of GSH significantly decreased(P<0.01);expressions of apopt osis proteins(Bax,active Caspase-3 and 9)were m arkedly increased,and the expression of the anti-apoptotic protein(Bcl-2)was d ramatically decreased(P<0.01);the expression of Prx II was significantly decreased(P<0.01).Compared with the m odel group,rutin significantly ameliorated those abnormalities caused by MI/RI injury,and markedly increased the endogenous antioxidant system and dose dependently.In addition,rutin significantly decreased the expression of apoptosis proteins(Bax,active Caspase-3 and 9),alonge with the increased expression of Bcl-2.The expression of Prx II was also significantly increased and dose dependently by rutin(P<0.01).Conclusion Rutin could reduce the m yocardial infarction area at the ons et of myocardial ischemia reperfusion injury in ra ts and improve myocardial systolic and diastolic function in a dose-dependent manner.Thus,rutin indeed possess the beneficial effect against myocardial ischemia reperfusion injury.The augmentation of Prx II expression may related to the cardioprotective effect of rutin.