| Background and Objective:Multiple myeloma is a common malignancy in the blood system,and the characteristics of this disease is that the malignant clonal proliferation by the plasmocyte. In recent years, more and more studies have shown that molecular genetic abnormalities are important factors, it can affect the prognosis of the MM.Therefore, how to accurately and quickly interpret these molecular and cytogenetic abnormalities has become the main research direction. Accompanied by Fluorescence in situ hybridization technology appear that improved the detection rate of MM cytogenetic abnormalities, which for clinicians to develop standardized and individualized treatment for MM patients,to provide a more accurate clinical basis.To investigate the effects and clinical meaning of fluorescence in situ hybridization?FISH? in the diagnosis and treatment of multiple myeloma ?MM? patients.Methods:Select of 100 MM patients whom were newly diagnosed at the first affiliated hospital of Zhengzhou University, from March 2015 to November 2016. All patients were examined by peripheral blood cells count, marrow cytology analysis,immunofixation electrophoresis, creatinine,serum calcium, C-reactive protein, ?2-microglobulin, X-ray and CT and other tests, including 65 males and 35 females;male to female ratio: 1.8: 1.By FISH, five specific probes including RB-1, P53,D13S319, IGH and 1q21 were used to study the chromosomal abnormalities of 100 newly diagnosed patients with MM in the First Affiliated Hospital of Zhengzhou University and analyze the correlation between the results and the patients, clinical indicators.Results:Among the 100 cases of newly diagnosed patients with MM, there were 60 cases of chromosomal abnormalities emerged and the overall detection rate of chromosomal abnormalities was 60%?60/100?. The frequencies of abnormalities in IGH rearrangement,lq21 amplification,the deletion of RB1, the deletion of D13S319 and that of P53 were 42% ?42/100?,32% ?32/100?,23% ?23/100?,21% ?21/100? and 5% ?5/100?, respectively. Analysis showed that there was a correlation between IGH gene rearrangement and the percentage of plasma cell ?>30%? as well as ISS periodization; one between del?13q? and ISS periodization, the percentage of plasma cell ?>30%? and that of ?-2 microglobulin ??2-MG?; and another between lq21 amplification and ?2-MG as well as ISS periodization. In addition, the detectable rate of the deletion of P53 was the lowest and the relevant prognosis may be worse. In a word, the chromosomal abnormalities in patients with MM mainly lie in IGH and stage IIIConclusions:1. The most common chromosomal abnormalities in patients with MM are IGH gene rearrangement and lq21 amplification; the second most common ones are the deletion of RBI and D13S319; the least one is the deletion of P53.2.There was a correlation between IGH gene rearrangement and the percentage of plasma cell?>30%? as well as ISS periodization; one between del?13q? and ISS periodization, the percentage of plasma cell ?>30%? and that of ?-2 microglobulin ??2-MG?; and another between lq21 amplification and ?2-MG as well as ISS periodization. In addition, the detectable rate of the deletion of P53 was the lowest and the relevant prognosis may be worse. |
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