Fluorescence In Situ Hybridization Technology And G-banding In The Study Of Multiple Myeloma

Objective1.To study the incidence of multiple myeloma patients with common chromosomal abnormalities.2.To study the relevance of the chromosomal abnormalities and laboratory examinations.3.The advantages and disadvantages of G-banding and FISH to detect chromosomal abnormalities in patients with MM.MethodWe have collected 16 cases of untreated multiple myeloma patients with bone marrow specimens,using interphase fluorescence in situ hybridization with probe 1q21 amplification,RB1 deletion,D13S319 deletion,P53 deletion and IGH rearrangement,And using G-banding technology to analysis karyotype of 16 cases of MM patients.Result1.The results showed that there were 12 patients with chromosome abnormalities in 16 cases of MM patients,the total incidence was 75%.The appearance of IGH rearrangement,13q deletion,1q21 deletion,and p53 deletion was 69%,50%(RB1 4380%;D13S319 25%),38%and 0.2.Serumβ₂-microglobulin,Intramedullary plasmacytosis and Bone loss are associated with IGH rearrangement;Serumβ₂-microglobulin,sex,ISS stage, Lactate dehydrogenase(LDH),Platelet(PLT),Phosphorus and Leukocyte abnormal are associated with RB1;Alanine aminotransferase(ALT),ISS stage, Serumβ₂-microglobulin and PLT are associated with D13S319;C-reactive protein(CRP),and age are associated with 1q21 amplification.3.Conventional cytogenetics(G-banding) results showed there were 5 patients with chromosoma abnormalities in 16 cases of MM patients,the total incidence was 31%,and with FISH technology the detection rate of MM chromosomal abnormalities was 73%.Conclusion1.IGH rearrangement happen early in the MM and been the highest rates for adverse prognostic factors.There is a wide range of deletion 13q,and it as independent poor prognostic factor,1q21 rearrangement also occurred in the stage of disease progression.There is a low rate of p53 mutation in MM.2.MM detection rate of chromosomal abnormalities by FISH technology was significantly superior than the traditional methods,but for uncertain chromosomal abnormalities,we need a combination with conventional cytogenetics.