Research Of Metabolic Characteristic Of Infantile Cytomegalovirus Hepatitis With Syndrome Of Spleen Deficiency With Dampness Encumbrance Based On GC-MS Technology

Objective:Study on the plasmic and urinary metabolic perturbation of Cytomegalovirus hepatitis with syndrome of spleen deficiency with dampness encumbrance to explore the essence of syndrome.Methords:Twenty cases of CMV hepatitis with syndrome of spleen deficiency with dampness encumbrance in line with diagnostic conditions were selected as disease group,while twenty cases of healthy children were recruited as control group.Plasma and urine were respectively collected as early as diagnosed and detected by gas chromatography-mass spectrometry.XCMS software was used for data extraction and data conversion while SIMCA-P software was used for multivariate statistics analysis.The corresponding mathematical model such as principal component analysis and orthogonal least discriminant analysis was established to identify the significantly altered metabolites and explore the essence of CMV hepatitis with syndrome of spleen deficiency with dampness encumbrance.The associated pathways were constructed using Metabo Analyst 3.0.Results:1.Extract of plasma metabolic profiling pattern analysis using PCA and OPLS-DA models exhibited clear separation for each group,which indicated the metabolic profiling in disease group were significantly changed when compared with control group.Twenty-two significantly altered metabolites were identified as potential biomarkers.Lactic acid,L-Alanine,Octanoic acid,L-Isoleucine,L-Proline,Glycine,Serine,L-Threonine,Amino-malonic acid,L-Methionine,Pyroglutamic acid,pentanoic acid,L-Glutamic acid,Ornithine,L-Lysine,L-Tyrosine,Myo-Inositol,Stearic acid,L-Tryptophan and Cholesterol in disease group were significalty increased when compared with control group,while 3-Hydroxybutyric acid and Edetic acid in disease group were significantly decreased.2.Extract of urine metabolic profiling pattern analysis using PCA and OPLS-DA models exhibited clear separation for each group,which indicated the metabolic profiling in disease group were significantly changed when compared with control group.Nine significantly altered metabolites were identified as potential biomarkers.Glycine,L-Threonine,L-Lysine,D-Mannitol,D-Gluconic acid,Palmitic Acid,Uric acid and Stearic acid in disease group were significalty increased when compared with control group.While Citric acid in disease group were significantly decreased.Conclusion:1.GC-MS platform adopted by this research can profile the samples of plasma and urine well,which also capable of differentiate and explaine the metabolic characterization of CMV hepatitis with syndrome of spleen deficiency with dampness encumbrance and normal healthy children.2.Perturbation of Glycine,serine and threonine metabolism,Arginine and proline metabolism,Alanine,aspartate and glutamate metabolism,Aminoacyl-tRNA biosynthesis,Lysine degradation,Pyruvate metabolism,Inositol phosphate metabolism,D-Glutamine and D-glutamate metabolism,Tryptophan metabolism and Lysine biosynthesis are mainly induced in CMV hepatitis with syndrome of spleen deficiency with dampness encumbrance.3.These selected different metabolites could be characterized potential biomarker of infantile cytomegalovirus hepatitis with syndrome of spleen deficiency with dampness encumbrance.