Protein Expression Profiling In Anterior Root And Posterior Root Of Spinal Nerve In Rat After Injury

Objective: The incidence of peripheral nerve is high. A series progresses such as the degeneration of axons and myelin sheath of the distal stump, the migration, proliferation and invasion of the inflammatory cells, which is called Wallerian degeneration. WD can harm the function of the nerve, while it plays an important role in nerve regeneration.Nerve misconnection often occurs in the progress of peripheral nerve regeneration. The motor nerve may target to the sensory organ, this may have connection with the protein difference in motor and sensory nerve. However, relative studies indicate that the differentially expressed proteins in motor and sensory nerve during WD play a more important role to the chemotactic regeneration of peripheral nerve. So we analyze the differentially expressed proteins in anterior root and posterior root of the spinal nerve, to provide relevant data support to chemotactic regeneration of peripheral nerve.Method: 60 adult female SD rats, weighing 220-250g. Anterior root and posterior root transection was performed in the right side. The left side is the control group. Seven days later, samples of the proximal stump of injured anterior root nerve, the distal stump of injured posterior root nerve, the normal anterior root nerve and the normal posterior root nerve were obtained. There were 8 samples in total, 2 in each group. iTRAQ was used to identify the differentially expressed proteins. GO and KEGG were used to analyze the differentially expressed proteins.Results: A total number of 4662 proteins were identified. (1)There are 17 differentially expressed proteins in the injured anterior root nerve (motor nerve) compared to the normal anterior root nerve (sensory nerve), in which 11 were up-regulated and 6 were down-regulated. (2)There are 2 differentially expressed proteins in the injured posterior root nerve compared to the normal posterior root nerve, in which 2 were down-regulated proteins. We find that there are no co-regulated differentially expressed proteins in the anterior root nerve and posterior root nerve 7 days after injury. The identified differentially expressed proteins are specific.Conclusion: We screen out differentially expressed proteins of anterior root and posterior root of spinal nerve seven days after injury, which provided data for the further study of peripheral nerve regeneration.