The Effect Of EGF On Regeneration Of Sweet Gland

ObjectiveTo study the effect of epidermal growth factor on regeneration of sweet gland.MethodsWe first prepared a bilayer collagen/chitosan scaffold,whose upper layer was the gelatin microspheres loaded with silver sulfadiazine(AgSD).Then the collagen/chitosan scaffold incorporated with bone mesenchymal stem cells(BMSCs)was treated as the control group,and the collagen/chitosan scaffold incorporated with both BMSCs and epidermal growth factor(EGF)was treated as the experiment group.The two groups were implanted into the bilateral feet of the SD-Rat with full thickness defect separately.The regeneration tissues were harvested and evaluated by HE and the immunohistochemical staining(IHC)at the time of 2w,4w and 8w to examine the structures of sweet gland.ResultsAs time went on,the wounds in both groups were healed.In the experiment group,the HE staining of paraffin section shown some incompact sweet gland structures at 8w after the surgery,while there were no sweet gland-like structures in the control group.The CK8 and CK14 by IHC shown positive at every point in time in both groups,but expression level was higher in the experiment group compared to the control group.The results illustrated that it could achieve the regeneration of epidermis and dermis with sweet gland in the experiment group,while it could achieve the regeneration of epidermis and dermis without the regeneration of sweet gland in the control group.Conclusion1.The collagen/chitosan scaffold being incorporated with BMSCs could not achieve the regeneration of sweet gland.2.The collagen/chitosan scaffold being incorporated with both BMSCs and EGF could not only achieve the regeneration of epidermis and dermis but also achieve the regeneration of sweet gland.3.Through inducing BMSCs differentiation into sweet gland cells,EGF can achieve the regeneration of sweet gland.