Sema4d And CXCR4 Expression In Lung Cancer Tissues And Its Relationship With Angiogenesis,Metastasis

Purpose:To investigate the expression of Sema4D,CXCR4 in lung cancer and to explore its relationship with prognosis and clinicopathological parameters of patients with lung cancer.Method:Immunohistochemistry was used to detect Sema4D,CXCR4 expression in 95 cases of non-small cell lung cancer tissues,and 20 cases of normal lung tissue specimens,analyzing the relationship between the clinical and pathological factors of lung cancer.Immunohistochemistry was used to detect expression of CXCR4 and microvessel density(MVD)value to analyze the relationship between the expression of CXCR4 and the MVD,as well as the relationship between lung cancer the relationship and pathological factors.RT-PCR was used to detect levels of CXCR4,comparing the results of immunohistochemistry test.Results:The expression Sema4D and CXCR4 in non-small cell lung cancer tissues was significantly higher than that in normal lung tissues(P<0.05),and its expression has nothing to do with gender,age,pathological type,differentiation of patients(P>0.05),but was associated with lymph node metastasis,TNM staging(P<0.05).There was no significant correlation between expression of CXCR4 and Sema4D in non-small cell lung cancer(P>0.05).The expression of CXCR4 and MVD value were significantly higher in Non-small cell lung cancer tissues than in normal lung tissues(P<0.01),the expression level of CXCR4 and MVD value were positively correlated(P<0.01).The results of CXCR4 expression in non-small cell lung cancer detected by immunohistochemical method and RT-PCR method were consistent.Conclusion:The clinical detection of Sema4D and CXCR4 expressions in tumor tissue from patients with non small cell lung cancer has positive significance in the study of lung cancer occurrence,development and prognosis,etc.,and may become a new target for treatment of non-small cell lung cancer.