Study On Antiviral Activity And Quality Standard Of Cinicifuga Dahurica

So,on the basis of this experiment in chemical separation,and related components of antiviral experiment,to evaluate the quality of different origin cohosh,established cohosh standard one fingerprint,provides the basis for further clinical study.First,this paper collected from made in China liaoning sing-an cohosh Cimicifuga foetida L.on the chemical composition and biological activity studies of the system.The research content mainly includes:the separation and purification of chemical composition and structure identification.By thin layer chromatography,LH-20 gel column chromatography,silica gel column chromatography,ODS column chromatography and reversed-phase high performance liquid chromatography(HPLC)extraction and purification methods,such as from cohosh Cimicifuga foetida 1.compounds isolated from the six monomers,cohosh glycol(1),25-O-acetyl cohosh alcohol-3-O-?-D-(2),wood indican cohosh alcohol-3-O-?-D-wood indican(3)(4),ferulic acid,ferulic acid and methyl ferulic acid(5)(6).Compounds isolated from the second,the mother liquor made from cell to maintain fluid 1:2-25 6 times than diluted to 96 hole in the cell culture plate has grown into a single layer,100 mu L/hole,each liquid dilution degrees do 4 hole,at the same time set up normal cells.Will cultivate board 37 ? and 5%C02 incubator in training,daily observed under inverted microscope cell pathological changes,no apparent cell changes in the minimum dilution ratio,at the same time set up normal cell and virus control.Finally,this paper established the sing-an cohosh standard medicinal broth of fingerprint,and the collected 10 DNA barcode molecular identification of batch cohosh medicinal materials,preparation of cohosh yinpian medicinal broth,and establish its quality standard.Medicinal materials of genomic DNA extraction cohosh,amplification and two-way sequencing sequence.All sequence CodonCode Aligner V 4.2 after joining together,through the NCBI-BLST site to identify the sequence alignment,10 batches of cohosh are sing-an cohosh,cohosh for the quality evaluation at the same time,according to the stipulations in the pharmacopoeia of the 10 batch cohosh the content of ferulic acid were determined,the results show that different content of ferulic acid in 0.204%0.400%,shall conform to the requirements of the pharmacopoeia,for qualified products.Through the preparation of the standard one method to establish the fingerprint of has paved the way for the quality evaluation of medicinal herbs and its preparations.