The Study Of Regulatory B Cells And Other Related B Lymphocytes In Peripheral Blood Of Patients With Multiple Sclerosis

Objective Through the detection of the percentage difference of regulatory B cells and related B lymphocyte in total B lymphocyte between multiple sclerosis patients and normal control group,and analyzing the correlation between different clinical severity of patients and regulatory B cells,we hope to investigate the potential role of B lymphocyte subsets in multiple sclerosis.Methods We collected the peripheral venous blood samples of multiple sclerosis patients who were hospitalized in the Department of Neurology in the First Affiliated Hospital of Bengbu Medical College from July 2016 to March 2017(n=12,male 8cases,female 4 cases,average age 41.42±4.53 years),and the health checkup people from Outpatient Department in the same period with no neurological or immune system disease(n=12,gender and age were matched with the former group).EDSS disability score for all patients with multiple sclerosis was conducted.Using flow cytometry to detect the percentage of CD19+CD24hi CD38 hi cells,CD19+CD27-CD38 hi cells and CD19+CD27hi CD38 hi cells in total B lymphocyte in peripheral venous blood of multiple sclerosis patients and control group,and the levels of IL-10 in peripheral blood of patients and controls were measured by ELISA.Record all data for statistical analysis.Results There were statistically differences in the percentage of regulatory B cells(CD19+CD24hi CD38hi)and transitional B cells(CD19+CD27-CD38hi)in total B cells and the level of IL-10 in peripheral blood between multiple sclerosis patients and the controls(P < 0.05),The percentage of regulatory B cells(CD19+CD24hi CD38hi)and transitional B cells(CD19+CD27-CD38hi)in total B cells and the level of IL-10 in peripheral blood of patients with multiple sclerosis were lower than in the controls. The percentage of plasma cells(CD19+CD27hi CD38hi)was not statistically significant compared with the control group(P >0.05).There was no significant difference in the expression rate of regulatory B cells in multiple sclerosis patients with different clinical severity EDSS score(P >0.05).Conclusion The expression rate of regulatory B cells and transitional B cells and the level of IL-10 in multiple sclerosis patients are lower than healthy person.B lymphocytes are involved in the pathogenesis of multiple sclerosis.Decreased expression of regulatory B cells may not be related to clinical severity of patients.