The Effects Of Long Non-coding RNA ATB On The Malignant Characteristics Of Human Glioma Cells

Objective To explore the biological functions of Long non-coding RNA ATB in glioma Methods Real-time PCR was used to detect the expression of ATB in 79 human glioma tissues and 15 normal brain tissues(from post-craniocerebral injury patients)and human glioma cell lines.We firstly designed an ATB knockdown model using transfection of sh-ATB plasmid in U251 and A172 glioma cell lines and its knockdown efficiency was remarkable compared with sh-control;Cell proliferation was examined by Cell Counting Kit-8(CCK-8)assay;The colony-forming ability was verified by colony formation assay;The invasion and migration ability were detected by the means of transwell and wound healing assay.Tumor xenograft formation assay was performed to evaluate the functional roles of ATB in vivo,Western blot and Ki-67 staining was performed to measure the proliferation ability in xenografted tumor tissues.Results The results of qRT-PCR showed that the expression of ATB in glioma tissues was significantly higher than that in normal human brain tissues,and the expression level of ATB increased with the malignant degree of glioma.After transfection of sh-ATB,as compared with sh-control group,the cell viability detected by CCK-8 assay and the clone numbers detected by colony formation assay both decreased.Knockdown of ATB expression in U251 cells and A172 cells significantly inhibited cell invasion and migration detected by the means of transwell and wound healing assay respectively.Knockdown of ATB significantly suppressed tumor growth in vivo,Tumor volumes in the sh RNA-ATB group were obviously smaller compared with the sh RNA-control group.Also,tumor weights in the sh RNA-ATB group were significantly lower than in the sh RNA-control group.Western blot and Ki-67 staining indicated that the PCNA protein and Ki-67 protein were both decreased in the sh RNA-ATB group compare to the sh RNA-control group,which suggested that sh RNA-ATB group had fewerproliferative cells than that in the sh RNA-control group.Conclusion In summary,we reported that ATB was highly expressed in glioma tissues and acted as an oncogene,which serves a key function in regulating glioma malignancy.ATB knockdown suppressed glioma biological characteristics both in vitro and in vivo,Our findings suggest that ATB thereby may represent a potential therapeutic target for the treatment of human glioma.