Expression Of CYP17A1 In Glioma Cell Lines And Effects Of Its Inhibitor On Proliferation Of Glioma Cells

Objective:To detect the expression of P450c17a(CYP17A1)in glioma cell lines T98 G,U87 and U251.And to investigate the effects of Abiraterone(CYP17A1 inhibitor)on proliferation of glioma cells.Method:(1)The expression of CYP17A1 in human glioma cell lines T98 G,U87 and U251 was detected by Western blot and Real-Time PCR.(2)T98G cells were cultured with different concentrations of abiraterone,Real-time PCR was employed to measure the mRNA expression of CYP17A1,Western blot was employed to measure the protein expression of CYP17A1,the proliferation of T98 G was detected by cck8 cell counting kit,Flow Cytometry was used to detect the apoptosis of T98 G.Results:(1)Western blot demonstrated that the expression of CYP17A1 was higher in T98G(0.518±0.052)and U251(0.460±0.034)than U87(0.142±0.025)(P<0.05).Real-time fluorescent quantitative PCR analysis indicated that the positive expression of CYP17A1 in T98 G,U87 and U251 cell lines were(1.000±0.122)?(0.960±0.079)?(0.611±0.045).CYP17A1 protein positive rate and m RNA levels in glioma cell lines T98 G and U251 were significantly higher than that of U87(both P <0.05).But CYP17A1 protein positive rate and m RNA levels were not significantly different between T98 G and U251(P>0.05);(2)After the treatment of abiraterone,T98 G displays a phenotype of apoptotic cells and the expression of CYP17A1 changed.The effect of apoptosis and expression both associated with concentrations of abiraterone.Conclusions:(1)CYP17A1 is highly-expressed in glioma cell lines T98 G and U251,which can be used as an ideal cell model for the research of the role of CYP17A1 in glioma and the specific therapies targeting CYP17A1.(2)These findings indicated that abiraterone inhibits cell proliferation.Promoted.cell apoptosis and the changed expression level of CYP17A1 could be partly due to its inhibitory in glioma cells.