Biological Significance Of CYP17A1 In Glioma

Objective To detect the expression of CYP17A1 in different histologic grade glioma tissues in vitro.and significance of CYP17A1 and AR co-expression was discussed.To establish a CYP17A1 gene silencing glioma cell line and explore the effects of CYP17A1 gene silence in glioma,which lay a foundation for the further researches about androgen pathway in glioma.Methods ?The expression of CYP17A1 in different histologic grade glioma tissues was detected by Immunohistochemistry?Western blot and Real time-PCR in 55 cases of different histologic grade glioma tissues and 10 cases of normal brain tissues.? The co-expression of CYP17A1 and AR was detected by Immunohistochemistry in different histologic grade glioma tissues.? the lentiviral vector which express the CYP17A1 sh RNA was constructed and CYP17A1 gene silencing glioma cell line was established.Then CYP17A1 m RNA in different groups were detected by Real time-PCR respectively.? The proliferation of CYP17A1 gene silencing glioma cell line was detected by CCK-8.Effects of migration and invasion about CYP17A1 gene silencing glioma cell line was performed by Transwell assay.Cell apoptosis was examined by flow cytometry.And nude mouse tumorigenicity assay was used to observe the effects of CYP17A1 gene silencing in vivo.Results ?Compared to normal brain tissues,The protein and m RNA expression of CYP17A1 gene in glioma was significantly higher,with statistical significance(P<0.05).?The expression of CYP17A1 protein and AR protein in glioma tissue samples is high than normal brain samples,and the expression of both proteins is related with histologic grades(P <0.05).? CYP17A1 gene silencing glioma cell line was established,And m RNA of CYP17A1 gene in T98G-CYP17A1 group was significantly decrease(F=15.44,P <0.05).?Compared to Vector-control or the Blank,the proliferation ?invasion?migration of T98G-CYP17A1 group revealed that T98G-CYP17A1 group showed a significant reduction in cell viability.Cell apoptosis of T98G-CYP17A1 group increased significantly.And the poor tumorigenic ability of T98G-CYP17A1 group was confirmed by tumorigenicity assay(P <0.05).Conclusions ? The protein and m RNA expression of CYP17A1 gene in glioma was significantly higher than the normal brain tissues.? The expression of CYP17A1 protein and AR protein was higher than normal brain tissues,which indicated androgen pathway takes part in generation and development of glioma.? CYP17A1 gene silencing glioma cell line was established successfully,which provided a base for further researches about regulation of CYP17A1 gene in glioma in vitro and vivo.?the proliferation ?invasion?migration of T98G-CYP17A1 group were significantly inhibited.And Cell apoptosis of T98G-CYP17A1 group increased significantly.CYP17A1 gene silencing can inhibit the viability of glioma cells in vitro and vivo.