| objective The effects of PRP on the local microenvironment after peripheral nerve rupture were studied by using the autologous platelet-rich plasma(PRP)at different time points to establish the peripheral nerve of the injured peripheral nerve.To analyze the relationship between peripheral nerve regeneration and local microenvironment parameters VEG and IGF-1,and to observe whether the localized administration of PRP to the regeneration of peripheral nerve in different time points will lead to continuous induction and promotion,and use macroscopic and ultramicroscopic And biochemical and other indicators to verify the feasibility of PRP on the treatment of peripheral nerve injury,analysis of its possible principles and mechanisms.To find a reasonable,economical,effective,easy treatment for the peripheral nerve injury.Methods: 1.Select 48 of the same origin,age and weight of the New Zealand white rabbits,male and female.Divided randomly into four groups: group A(sham operation group),group B(NS group),group C(S-PRP group),group D(T-PRP group)2.Animal model establishment: 2.1 C,D two groups of experimental rabbits with anticoagulant syringes to extract ear vein blood preparation PRP with Landesberg two centrifugation,and platelet detection.2.2All the experimental animals: aseptic operation,experimental rabbits dissecting sciatic nerve,A group only exposed,B,C,D group in the lower edge of papillary muscle about 1.5cm completely cut off,and then 8-0 micro-suture uniform suture 4 needle.2.3 Group B in the suture and around the injection of NS 0.4ml,C,D group in the nerve suture at the end and around the injection of PRP 0.4ml(C,D group before surgery with the above method to prepare PRP spare),Stitching.Postoperative rabbits non-experimental side of the buttocks intramuscular antibiotics to prevent infection treatment for 3 days,limiting part of the activities,the same living environment and feeding methods.3.The rats in group B were given 0.4 ml NS at the periphery of sciatic nerve suture and 0.4ml PRP was injected into the periphery of sciatic nerve suture.The rats in group D were treated with whole blood before operation,Preparation PRP stand-by).4.Observation indicators: 4.1 12 weeks after surgery,with the modeling method of dissecting all animal sciatic nerve,the electrophysiological examination.4.2 After electrophysiological measurement,cut the sciatic nerve fracture suture nerve section,longitudinal section open,of which 1/2 to do the specimen fixed,embedding,making slices,light microscopic observation of regeneration;and immunohistochemical staining,observation of S-100 Protein expression,recording Schwann cell activity.4.3 The other 1/2 nerve segment Western Blot recorded VEGF,IGF-1 expression results.4.4 Surgical separation of gastrocnemius,cut about 0.1g muscle tissue for AChE content detection.5.SPSS 24.0 statistical software package for statistical analysis of the data.6.Experimental standards refer to international or domestic authority of the relevant instruments.Results: 1.The average platelet concentration in the whole blood was 304.87 × 109 / L,the average platelet concentration in PRP was 1336.42 × 109 / L,and the platelet count in PRP was 4.38 times of that of whole blood.2.The sciatic nerve potential(CNAP)was superior to NS group and S-PRP group in T-PRP group at 12 weeks postoperatively(P <0.05).3.The cytokines VEGF and IGF-1 were positive in each group at 12 weeks postoperatively.The results showed that the optical density of VEGF and IGF-1were significantly higher in the T-PRP group than in the other three groups Statistically significant(P <0.05).4.The AChE content in the gastrocnemius muscle of the sham operation group and the T-PRP group was significantly higher than that of the NS group and the S-PRP group(P <0.01).The NS group and the S(P <0.01).There was no significant difference between the two groups(P <0.01).There was no significant difference between the two groups(P <0.01).5.The content of S-100 protein in the sham operation group,NS group and S-PRP group was significantly lower than that in the T-PRP group at12 weeks after operation(P <0.01).There was no significant difference between NS group and S-PRP group(P> 0.05).Conclusion: 1.PRP can increase the content of VEGF and IGF-1 in the sciatic nerve,and promote the division and proliferation of Schwann cells by applying PRP at the local time point of rabbit sciatic nerve.2.Multi-frequency nerve fracture area injection PRP,can promote the synthesis and release of cholinergic neurotransmitter ACh,can enhance the peripheral nerve injury after exercise end plate repair,is conducive to the regeneration of peripheral nerve repair and functional recovery. |
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