Study On The Effect And Mechanism Of The Double-Ginseng Particle Invigorating Spleen And Hidroschesis

Pseudostellaria heterophylla(short for PHP below)is from Caryophyllaceae perennial herbaceous plants Pseudostellaria heterophylla(Miq.)Pax ex Paxethoffm.It has the efficiency of supplementing and tonify spleen deficiency and promoting production of body fluid and nourishing the lung,which can be used to treat spleen deficient,loss of appetite,pediatric weak,indigestion,weak after illness,qi and Yin deficiency,spontaneous perspiration thirsty phlegm-heat type and dry cough,etc in clinical.Wahlenbergiamar ginata(short for WMG below)was from Campanulaceae blue flowers and plants of the genus and blue Wahlenbergiamar ginata(Thunb.)a.DC.Its radix and whole herb medicine can be used as medicine.It has the efficiency of tonify deficiency spleen,tonify deficiency,spontaneous sweating,night sweats,in addition to heat,cough expectorant effect and so on.Suanshen granule(short for SSG below)is combine PHP from genuine Chinese medicine materials of Fujian province and WMG of China green grass drug,which is under the guidance of traditional Chinese medicine theory to reasonable formula.The compatibility altogether of these medicine plays insupplementing and tonify spleen deficiency,which can be used to treat spontaneous sweat caused by qi and spleen deficiency.Combined with modern preparation methods of the preparation process,these two drugs were make a kind of a granule which effect is good,easy taking,good adherence spontaneous perspiration and good safety management.Its raw material sources,simple preparation process and low cost.In order to further develop the double and tonify deficiency spleen hidroschesis particles hidroschesis product,provide theoretical basis for its clinical application.These researches develop its pharmacodynamics and the mechanism of molecular biology of SSG.1.Water extract-alcohol precipitation using the method,the invention patent(ZL201310018999.8)with reference to previous research,preparation of yellow brown granules,using vanillin ice acetic acid color and uv spectrophotometry determination of total extract and the content of total saponins in the granules were 0.2419%and 0.2386%respectively;Using phenol sulfuric acid color and ultraviolet spectrophotometric method for determining the content of total polysaccharide extract was 11.56%2.The COPD rats model were made by double elements of smoking and douching Mahuang Decoction.Rats were intragastric administration(i.g.)withMahuang Decoctionat 1st,7th,14th day after 45min i.g of SSG.Hetian-gaohereagent A was smeared at the ministry of foot unable.Reagent B was smeared after reagent A was dried.Within 15 minutes,the amount of modena point(sweat point)was counted by magnifying glass of 10 times.As a result,compared with control group the model group has a significant difference.Hidroschesis effect was not evidence at 1st and 7th day,but at the date of 14,the administration groups(The sweat points in the high and medium dose groups were 26.0,29.0)had significant difference compared with control group(The perspiration count is 38.7).3.The spleen vacuity spontaneous sweating rats model were induced byreserpine.Rats were injected with pilocarpine at 1st,7th,14th day after 45min i.g of SSG.Hetian-gaohe reagent A was smeared at the ministry of foot unable.Reagent B was smeared after reagent A was dried.Within 15 minutes,the amount of modena point(sweat point)was counted by magnifying glass of 10 times.As a result,compared with control group the model group has a significant difference.Hidroschesis effect of was not evidence at 1st day,the administration groups(The sweat scores in the high,medium and dose groups were 62.5,64.7)had significant difference compared with control group(The perspiration count is 93.6).Low dose group the difference was not significant.4.Using Western blot method-detection of AChR protein expression content in brain tissue samples.As a result,the dosage of AchR dose groups of rats had compared with model group,the protein expression of grayscale values have significant difference.Double parameter can particles can inhibit in model rats had AchR protein expression.5.The Real-Time PCR method was used to detect tissue samples of rats mRNA gene transcription level changes,as a result,the dosage of AchR dose groups of rats had compared with model group,the amount of gene expression have significant difference.Double and particles can reduce the model in the rat brain neurotransmitter relative expression of AChR gene.Based on double and tonify deficiency spleen stop sweat the effects of the particles and the mechanism of hidroschesis experimental study,the results show that the double and tonify deficiency spleen qi deficiency anti-perspirant particles can inhibit spontaneous perspiration rats and the role of spontaneous sweat rats sweat pixu(spleen deficient),and its preliminary mechanism is by inhibiting AchR protein expression in brain tissue and brain neurotransmitter AchR,relative expression of genes for hidroschesis product development and provide theoretical basis for clinical application.