Experimental Study Of Xiaoyao Hollow Suppository On The Behavior Of Rats And The Sex Hormone Of The Liver Type

1 research purposesThe purpose of this experiment was to compare from animal behavior and sex hormones E2,T and PRL in two aspects of Xiaoyao Powder Free Decoction and Xiaoyao hollow suppository treatment effect,explore the traditional Chinese medicine theory under the guidance of two forms of rats with liver qi stagnation of the treatment effect,for clinical rational use of Xiaoyao Powder to provide support.2 research methodologyIn January at the age of 50 female SD rats were randomly divided into 5 groups: control group,depression model group,Xiaoyao Decoction group,Xiaoyao granules hollow suppository high dose group,low dose Xiaoyao hollow suppository group,10 rats in each group,through a variety of chronic unpredictable stress depression stimulation random combination of Ganyu model method copy,21 days after each vaginal smear for 5consecutive days,at the same time do behavioral testing,free hollow bolt of high and low dose of anus nano medicine;Xiaoyao Powder Free Decoction group orally administered continuously for 10 days,after the end of the day to delivery a vaginal smear for 5 days,and at the same time do behavior the animals were killed after animal testing,on the second day,blood reserve.3 research content3.1 general state,weight,feedingAfter the experiment,the general state of rats was observed every day.The body weight and food intake were recorded every 3 days.3.2 vaginal smear testAfter the model wasad me,the rats were examined by vaginal smear for 5 days,and the estrous cycle was observed by microscope.3.3 behavioral testingRespectively after modeling and administration after the open field test,the elevated plus maze test,sucrose preference test,test the success of modeling and administration effectiveness.3.4 sex hormone testThe blood from abdominal aorta was centrifuged and the serum was separated.The contents of E2,T and PRL in serum were determined by radioimmunoassay.3.5 statisticsData were analyzed using SPSS17.0 software,the measurement data to mean +standard deviation,comparison between groups using independent sample t test,were compared with paired t test,analysis of variance was used to compare quantitative data between groups,P < 0.05,the difference was statistically significant.4 Results4.1 the successful model of decision: compared with the blank control group,model group,mean free Decoction group,Xiaoyao Xiaoyao hollow suppository high dose group,low dose group of Xiaoyao hollow suppository of general status,body weight,feeding behavior and the observation of a significant change in liver after modeling,analysis by t test,there was statistical the significance of P is less than 0.05 difference,suggesting that the model was successful.4.2 Observation ofgroups of vaginal smears: model control group after 5 days of smear in proestrus,estrus,estrous period of rest interval,alternately,only 1 days under the microscope showed typical keratinized cells increased;Ganyu model group was 5 days in3-5 days consecutive keratinocytes,Xiaoyao Decoction the granule group,Xiaoyao hollow suppository high dose group,low dose group of Xiaoyao hollow suppository after administration for 5 days in the cell under the microscope angle law has appeared,some have been restored to normal estrous cycle.4.3 through the analysis of behavioral data: in the open field test,from the peripheral and central lattice lattice squares crossed,Xiaoyao Powder Free Decoction group,Xiaoyao hollow suppository high dose group,low dose group and Xiaoyao hollow suppository in Ganyu model group compared to P < 0.05,there was statistical significance from the number of standing and physical;wool modification times for free hollow suppository high dose group P < 0.05,the difference was statistically significant;the elevated plus maze test,the open arm time percentage,Xiaoyao Powder Free Decoction group,Xiaoyao hollow suppository high dose group,low dose group and Xiaoyao hollow suppository of liver depression model group compared with P < 0.05.Statistical significance;sucrosepreference test,Xiaoyao Powder Free Decoction group,Xiaoyao hollow suppository high dose group,low dose group of Xiaoyao hollow suppository sucrose preference degree and Ganyu model group compared to P < 0.05,the remaining group was statistically significant;Statistical analysis of P > 0.05,no difference between the two groups.4.4 based on the sex hormone E2,T,PRL detection results: Xiaoyao Powder Free Decoction group,Xiaoyao hollow suppository high dose group T,PRL and liver of model group compared to P < 0.05,there was statistical significance;Xiaoyao hollow suppository low dose group PRL with Ganyu model group compared to P < 0.05,there was statistical significance;the comparison between groups by statistical analysis P > 0.05,that there is no difference.5 Conclusion5.1 the rats of various stress 21 days chronic unpredictable depression stimulation random combination method,can successfully replicate the Ganyu model.5.2 Ganyu model female rats with poor general condition,weight loss,decreased food intake,estrous cycle disorder,open field test in the horizontal and vertical score decreased the elevated plus maze test in the open arm time and frequency ratio decreased,sucrose preference decreased.5.3 happy hollow suppository can improve the liver qi stagnation syndrome in female rats general state,weight gain,food intake increased,raised in the open field test of the horizontal and vertical score,elevated plus maze open arm time and frequency ratio,sucrose preference degree and improve the disorders of estrous cycle,serum E2,T regulation and PRL content.Free decoction had no significant differences compared with Xiaoyao Powder,can be used as a new dosage form for clinical application.