The Protect Effect Of Ginsenoside Re For Vascular Dementia Rats MT From Chronicity Ischemic

Object:To investigate the protective effect of Ginsenoside Re on the mitochondrias from rats with chronic ischemia-induced VD,and provide experimental datas for developing new drugs which can safely and effectively prevent vascular dementia from ischemia.Method:Therat model of vascular dementia was involved permanent bilateral occlusion of the common carotid arteries.The protective effect of Ginsenoside Re was measured by Morris water maze and HE staining.Isolated mitochondria and protein by corresponding kits.Using bioelectron microscopy,western bolt and H₂O₂ kit to determine the difference between groups.Establish hypoxia-reoxygenation model by vacuum pump and cell incubator.Measured effect of the model in terms of the production of actase dehydrogenase?LDH?,the extent of DNA breakage and cell morphology.Assessed the production of the LDH and the extent of DNA breakage and observed cell morphology after drug action.And then isolated mitochondria and protein from hippocampal of rats of all groups.Using western bolt and H₂O₂ kit to determine the difference between groups.Results:After permanent bilateral occlusion of the common carotid arteries,the cognitive ability has decreased andthe latency time has obviously prolonged.The neurons of hippocampus from rats has been arrangedin disorder and shown vacuolization around cells,which indicated the neurons had lost the normal structures.The results of the cells ultrastructures,the expressions of COX IV and PDH-A1,as well as the rates of H₂O₂ production have shown that the Ginsenoside Re in different concentrations could not only contribute to the expression of COX IV and PDH-A1,but prevent the rates of H₂O₂ productionto some extent.The Ginsenoside Re could repair the damage of mitochondrias of hippocampneurons.There were significant increase in the production of LDH and the extent of DNA breakage,neuron degeneration and cell constraction of model group after hypoxia reoxygenation.Re could effectivly inhabit LDH release,DNA breakage and synaptic degeneration,and decrease the production of H₂O₂ but congtribute to the expression of COX IV and PDH-A1.Conclusion:The Ginsenoside Re could improve the cognitive ability of rats by protecting mitochondrias of hippocampneurons from Cerebral ischemia.Re effectivly protected hippocampal neurons from the damage of hypoxia-reoxygenation by improving the state of mitochondria.