Study Of Qihuang Bufei Decoction On Airway Remodeling And Cytochrome C In Asthmatic Rats

Objective:To observe the curative effect of Qihuang Bufei Decoction on airway remodeling in asthmatic rats,to reveal its mechanism through the activation of Cyt-C promotes apoptosis of ASMC.Methods:40 SD rats were randomly divided into blank negative control group,the asthma model positive control group,Qihuang Bufei Decoction intervention group and montelukast intervention group.10 rats in each group.The OVA sensitized 1%OVA solution,inhalation of 30min/times,3 times a week for 12 weeks.24h after the last challenge,the pulmonary tissue pathological sections for hematoxylin EOSin staining(HE staining)were observed;the use of computer image analysis software to measure the area of bronchial smooth muscle(Wam),bronchial basement membrane perimeter(Pbm),the total area of the bronchial wal(Wat);Determination of white blood cell(WBC)and EOSinophil(EOS)count in bronchoalveolar lavage fluid(BALF)by cell counter;Enzyme linked immunosorbent assay(ELISA)method for the detection of cytochrome C in airway smooth muscle homogenate(Cyt-C),in situ end labeling method(TUNEL method)to detect airway smooth muscle cells(ASMC)apoptosis index.Result:1.Qihuang Bufei Decoction intervention group and montelukast intervention rats in group BALF and WBC the number of percentage of EOS decreased significantlyt(P<0.01);2.Qihuang Bufei Decoction intervention group and montelukast intervention Cyt-C rats ASMC content in cytoplasm increased significantly,there was statistical significance compared with the asthma model positive control group(P<0.01),Qihuang Bufei Decoction intervention group Cyt-C content than Singulair intervention group was statistically significant(P<0.05);3.Qihuang Bufei Decoction intervention group and montelukast intervention group rat ASMC apoptosis index increased significantly,Compared with the positive control group,the difference was significant(P<0.01),Qihuang Bufei Decoction intervention group the apoptosis rate of ASMC is better than that of Singulair intervention group,the difference was statistically significant(P<0.05);4.Qihuang Bufei Decoction intervention group and montelukast intervention group rats Wam/Pbm,Wat/Pbm decreased significantly and significant compared with asthma model positive control group(P<0.01).Conclusion:Qihuang Bufei decoction can inhibit the asthma model positive control group rat airway remodeling,this inhibition may be related to mitochondrial apoptosis by activating protein Cyt-C,promote AMSC apoptosis,and inhibit the occurrence of airway remodeling.