| Objective:Based on the changes of monoamine neurotransmitters in the nucleus accumbens,the mechanism of improving the sleep by wild jujube kernel freeze dried powder was studied.Method:1.Under the LD condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours10 male SD rats were selected,under anesthesia and stereotaxic localization of the brain,the microdialysis cannula was implanted in the basal forebrain accumbens region of the rat.After the operation was completed,under LD conditions(12 hours/12 hours,alternation of light and shade),the experimental animals resumed 2 days.It begins at 7:00 on the third th,until 5:00 the next day,Microdialysis technique is utilized,every 2 hours,take experimental animal brain dialysis solution for 1 hours.Then,use HPLC-EC to detect the contents of NE,DA and 5-HT in the dialysate of the experimental animals.The content of sleep-wake cycle(24 hours)was observed.2.Under the LL condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours10 male SD rats were selected,under anesthesia and stereotaxic localization of the brain,the microdialysis cannula was implanted in the basal forebrain accumbens region of the rat.After the operation was completed,under LL conditions(24 hours of continuous illumination),the experimental animals resumed 2 days.It begins at 7:00 on the third th,until 5:00 the next day,Microdialysis technique is utilized,every 2 hours,take experimental animal brain dialysis solution for 1 hours.Then,use HPLC-EC to detect the contents of NE,DA and 5-HT in the dialysate of the experimental animals.The content of sleep-wake cycle(24 hours)was observed.3.Under the LL condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours with wild jujube kernel freeze-dried powderSelect ten male SD rats and give wild jujube kernel freeze-dried powder by gavage with 56g/kg last for 5 days.On the fifth days,anesthetize the experimental animals and place microdialysis cannula in the basal forebrain nucleus accumbens region of rats with brain stereotaxic.After surgery,recover for 2 days under the LL condition(24 hours of continuous illumination),in the meantime,give wild jujube kernel freeze-dried powder by gavage everyday.From 7:00 am on the third day to 17:00 on the fourth day,every two hours,take the brain dialysis solution of experimental animals for 1 hour by microdialysis automatically.Then,detect the content of the NE,DA and 5-HT in brain dialysis solution of the experimental animals by HPLC-Electrochemistry and observe the content change of awakening-sleep cycle(24 hours).Result:1.Under the LD condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours1.1 Under the LD condition,changes of NE content in the nucleus accumbens of rats in 24 hoursThe results revealed that the NE content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LD condition.1:00?2:00 sample content is higher,19:00?20:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 5.87±.21,10:00?11:00 is 4.56±0.41,13:00?14:00 is 5.90±0.52,16:00?17:00 is 4.98±0.40,19:00?20:00 is 3.88±0.32,22:00?23:00 is 5.56±0.38,1:00?2:00 is 6.92±1.14,4:00?5:00 is 4.65±0.55.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00,comparing the content of each two sampling points,there is a significant difference between them(P<0.05).1.2 Under the LD condition,changes of DA content in the nucleus accumbens of rats in 24 hoursThe results revealed that the DA content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LD condition.And the night content is higher than the daytime.The content of night showed a downward trend,19:00?20:00 sample is higher,4:00?5:00 sample content is lower;Daylight content is on the rise,16:00?17:00 sample is higher,7:00?8:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 3.47±0.77,10:00?11:00 is 7.81±0.91,13:00?14:00 is 9.51±0.86,16:00?17:00 is 10.31±0.69,19:00?20:00 is 12.38±1.05,22:00?23:00 is 10.71±0.71,1:00?2:00 is 8.39±0.64,4:00?5:00 is 6.46±1.20,Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;16:00?17:00 and 4:00?5:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).13:00?14:00 and 1:00?2:00,comparing the content of two sampling points,there is a significant difference between them(P<0.05).1.3 Under the LD condition,changes of 5-HT content in the nucleus accumbens of rats in 24 hoursThe results revealed that the 5-HT content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LD condition.And the night content is higher than the daytime.Among them,the content of 22:00?23:00 at night is higher,and 4:00-5:00 sample is lower;during the day,the content of 7:00-8:00 sample is higher,and 13:00?14:00 sample is lower.Concretely show:The content of 7:00?8:00 is 16.23±1.60,10:00?11:00 is 13.67±0.83,13:00±14:00 is 11.78± 1.02,16:00?17:00 is 15.43±0.58,19:00?20:00 is 18.42± 1.28,22:00?23:00 is 22.68± 1.42,1:00?2:00 is 18.60± 1.50,4:00?5:00 is 16.96±0.84.Among them,10:00?11:00 and 22:00?23:00;13:00?14:00 and 1:00?2:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).7:00?8:00 and 19:00?20:00;16:00?17:00 and 4:00?5:00,comparing the content of each two sampling points,there is a significant difference between them(P<0.05).2.Under the LL condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours2.1 Under the LL condition,changes of NE content in the nucleus accumbens of rats in 24 hoursThe results revealed that the NE content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition.1:00?2:00 sample content is higher,19:00?20:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 5.87±0.67,10:00?11:00 is 4.59±0.38,13:00?14:00 is 5.90±0.51,16:00?17:00 is 4.98±0.63,19:00?20:00 is 3.88±0.71,22:00?23:00 is 5.46±0.52,1:00?2:00 is 6.92±0.90,4:00?5:00 is 4.65±0.98.Among them,7:00?8:00 and 19:00?20:00,comparing the content of two sampling points,there is a very significant difference between them(P<0.01).2.2 Under the LL condition,changes of DA content in the nucleus accumbens of rats in 24 hoursThe results revealed that the DA content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition.And the night content is higher than the daytime.The content of night showed a downward trend,19:00-20:00 sample is higher,4:00-5:00 sample content is lower;Daylight content is on the rise,16:00?17:00 sample is higher,7:00?8:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 2.41±0.52,10:00?11:00 is 4.46±0.85,13:00?14:00 is 8.09±1.01,16:00?17:00 is 8.73±0.91,19:00?20:00 is 11.71±1.11,22:00?23:00 is 8.47±0.93,1:00?2:00 is 6.92±0.61,4:00?5:00 is 6.23±0.87.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;16:00?17:00 and 4:00?5:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).2.3 Under the LL condition,changes of 5-HT content in the nucleus accumbens of rats in 24 hoursThe results revealed that the 5-HT content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition.And the night content is higher than the daytime.The content of night showed a downward trend,19:00?20:00 sample is higher,4:00?5:00 sample content is lower;the content of the day decreased first and then increased,16:00?17:00 is higher,10:00?11:00 is lower.Concretely show:The content of 7:00?8:00 is 8.66± 1.44,10:00?11:00 is 7.39±1.41,13:00?14:00 is 8.89±1.29,16:00?17:00 is 10.35±0.86,19:00?20:00 is 13.02±1.12,22:00?23:00 is 12.52±10.66,1:00?2:00 is 12.03±1.07,4:00?5:00 is 9.45± 1.75.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;13:00?14:00 and 1:00?2:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).3.Under the LL condition,the research on the changes of monoamine neurotransmitter in the nucleus accumbens region of rats in 24 hours with wild jujube kernel freeze-dried powder3.1 Under the LL condition,the effect of wild jujube kernel freeze-dried powder on the content of NE in the nucleus accumbens area of 24 hours ratsThe results revealed that the NE content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition with wild jujube kernel freeze-dried powder intervene.1:00?2:00 sample content is higher,19:00?20:00,4:00-5:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 4.08±0.90,10:00?11:00 is 3.05±0.81,13:00?14:00 is 4.07±0.95,16:00?17:00 is 2.82±0.55,19:00?20:00 is 2.63±0.75,22:00?23:00 is 3.20±0.69,1:00?2:00 is 4.32±0.64,4:00?5:00 is 2.63±0.81.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;16:00?17:00 and 4:00?5:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).3.2 Under the LL condition,the effect of wild jujube kernel freeze-dried powder on the content of DA in the nucleus accumbens area of 24 hours ratsThe results revealed that the DA content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition.And the night content is higher than the daytime.The content of night showed a downward trend,19:00?20:00 sample is higher,4:00?5:00 sample content is lower;Daylight content is on the rise,16:00?17:00 sample is higher,7:00?8:00 sample content is lower.Concretely show:The content of 7:00?8:00 is 1.83±0.60,10:00?11:00 is 2.86±0.59,13:00?14:00 is 3.74±0.84,16:00?17:00 is 4.40±0.80,19:00?20:00 is 5.48± 1.01,22:00?23:00 is 4.21 ±0.85,1:00?2:00 is 3.94±0.76,4:00?5:00 is 2.24±0.72.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;16:00?17:00 and 4:00?5:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01).3.3 Under the LL condition,the effect of wild jujube kernel freeze-dried powder on the content of 5-HT in the nucleus accumbens area of 24 hours ratsThe results revealed that the 5-HT content change in AcbC region of the basal forebrain in rats have a certain fluctuation in 24h day and night under the LL condition.And the night content is higher than the daytime.The content of night showed a downward trend,19:00?20:00 sample is higher,4:00?5:00 sample content is lower;the content of the day decreased first and then increased,16:00?17:00 is higher,10:00?11:00 is lower.Concretely show:The content of 7:00-8:00 is 8.16±0.68,10:00?11:00 is 6.20±0.49,13:00?14:00 is 7.43±0.85,16:00?17:00 is 9.02±0.97,19:00?20:00 is 10.37± 1.02,22:00?23:00 is 11.17±0.83,1:00?2:00 is 9.05±0.79,4:00?5:00 is 8.31 ±0.72.Among them,7:00?8:00 and 19:00?20:00;10:00?11:00 and 22:00?23:00;13:00?14:00 and 1:00?2:00,comparing the content of each two sampling points,there is a very significant difference between them(P<0.01). |
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