Studies On The Saponins From Hylomecon Japonica(Thunb.) Prantl Et Kündig(?)

Background:Hylomecon japonica(Thunb.)Prantl et Kündig,a perennial herbaceous plant,belongs to Hylomecon genera,the family of Papaveraceae,widely distributed in the northeast,east and middle regions of China.The roots or whole plants are often used as traditional Chinese medicine to treat rheumatism and bruises for a long history.Saponins are deeply studied for many years,many structures of which are clear,having been reported to have activities of anti-cancer,anti-virus,anti-oxidation and so on.A variety of saponins have been used clinically.Objective:In previous studies on phytochemistry and pharmacology of Hylomecon genera,only several alkaloids and their related activities were reported,whereas there were no studies on saponins of it.However,studies on chemical constituents are material basis of application of traditional Chinese medicine.In order to develop and make full use of this plant,screen compounds with good pharmacological activities and finally be used clinically,this paper studied saponins from Hylomecon japonica(Thunb.)Prantl et Kündig.Methods:1.Extraction of saponins from Hylomecon japonica(Thunb.)Prantl et Kündig: The dried plants were percolated with 70% Et OH for seven days and repeated three times.The extraction was concentrated to remove Et OH.The Et OH extract of the plant was chromatographed on a D101 column.Collect 50% Et OH eluate,dry it under reduced pressure,getting crude saponins 337.3g.2.Isolation and purification of the saponins: The 50% Et OH eluate was subjected to HP-20 column chromatography.Collect 35% Et OH eluate,then use thin layer chromatography preparation and HPLC to get pure compounds.Results:Four pure saponins from Hylomecon japonica(Thunb.)Prantl et Kündig were obtained.Their structures were elucidated on the basis of spectral analysis and comparison with reported data as follows:(?)3-O-?-D-galactopyranosyl-(1?2)-?-D-glucuronopyranosyl-gypsogenin-28-O-?-D-xylopyranosyl-(1?2)-?-D-xylopyranosyl-(1?4)-?-L-rhamnopyranosyl-(1?2)-?-D-quinovopyranoside(?)3-O-?-D-galactopyranosyl-(1?2)-?-D-glucuronopyranosyl-quillaic acid-28-O-?-D-xylopyranosyl-(1?2)-?-D-xylopyranosyl-(1?4)-?-L-rhamnopyranosyl-(1?2)-?-L-arabinopyranoside(?)3-O-?-D-galactopyranosyl-(1?2)-?-D-glucuronopyranosyl-gypsogenin-28-O-?-D-xylopyranosyl-(1?2)-?-D-xylopyranosyl-(1?4)-?-L-rhamnopyranosyl-(1?2)-?-L-arabinopyranoside(?)3-O-?-D-galactopyranosyl-(1?2)-?-D-glucuronopyranosyl-gypsogenin-28-O-?-D-xylopyranosyl-(1?4)-[?-D-glucopyranosyl-(1?3)]-?-L-rhamnopyranosyl-(1?2)-?-L-arabinopyranoside Conclusion:Compound ?,? are newly isolated from natural sources,and Compound ?,? are isolated for the first time from this plant.