Study On The Effect Of Low Dose BMOV On Diabetic Rats Liver And Oxidative Stress

Objective:As a potential drug for diabetes,BMOV has been shown an activity of good reducing blood glucose.Because BMOV contains vanadium metal,its side effects have not been fully elucidated.The effects beyond reducing blood glucose need further study for diabetic rats.Oxidative stress is an important risk factor for diabetic complications.At present,there are few studies on the oxidative stress and BMOV in diabetic rats.what is more,the liver is the main organ of glucose and lipid metabolism and oxidative stress.The effects of BMOV on the liver and oxidative stress of diabetic rats should be further studied.This study was to investigate the effect of BMOV on liver and oxidative stress in diabetic rats,and to provide a reference for further study of BMOV.Method:In this study,we used 60 healthy male Wistar rats with weighing 200-220 g.we randomly selected 50 rats to inducing diabetes mellitus model,and the model of diabetic rats was successfully established with random blood glucose ?16.7 mmol / L.They were randomly divided into six groups: BMOV0.25 mg group(0.25 mg / kg / d),BMOV0.5mg group(0.5mg / kg / d),BMOV1 mg group(1mg / kg / d),glibenclamide group(1.5 mg / kg / d glibenclamide),diabetes mellitus(equal dose of saline),normal control group(equal dose of saline)for gavage,continuous for 3 weeks.Blood glucose and weight of rats was measured weekly.When the experiment finish,we collected serum and liver of rats.We detect the levels of serum glycosylated serum protein,triglyceride,cholesterol,low density lipoprotein,high density lipoprotein,superoxide dismutase,glutathione peroxidase,malondialdehyde,alanine aminotransferase,aspartate Transaminase level,body length,liver weight and other indicators.Hematoxylin-eosinstaining was used to observe the morphology of liver tissue.The expression of NF-?B and TNF-? was detected by immunohistochemical staining.Result:(1)BMOV0.25 mg group,0.5mg group,1mg group,glibenclamide group and diabetes group,the body weight and body length were lower than the normal control group(P<0.05).There was no significant difference among the six groups on lees' s index(P> 0.05).(2)The blood glucose of BMOV0.25 mg,0.5mg,1mg and glibenclamide groupsshowed a downtrend,while glycemic of the diabetic group did not decline.Glycosylated serum protein of BMOV0.25 mg,0.5mg,1mg group,glibenclamide group and diabetes mellitus group were higher than the normal control group(P<0.05).(3)There was no significant difference about serum triglyceride,cholesterol,low density lipoprotein,LDL and HDLamong the six groups(P> 0.05).(4)There was no significant difference in serum super oxide dismutaseamong the six groups(P>0.05).The activity of glutathione peroxidase(GSH-Px)in BMOV 0.25 mg group and 0.5mg group was higher than that in diabetic model group(P<0.05),but there was no significant difference between positive control group and normal control group(P<0.05),The serum level of GSH-Px in BMOV 1mg group was lower than that in the positive control group and normal control group(P<0.05)The level of serum malondialdehyde(MDA)was lower than that of diabetes mellitus group(P <0.05).The level of serum MDA in BMOV 0.5mg group and 1mg group was higher than that in normal control group(P<0.05).(5)There was no significant difference in serum alanine aminotransferase and aspartate aminotransferase among the six groups(P> 0.05).Compared with normal liver tissue,0.25 mg,0.5mg,1mg,glibenclamide and diabetic rats had fat vacuoles and glycogen vacuoles.BMOV 1mg group observed the presence of inflammatory cell invasion.For five test groups,the livers have different degrees of injury.(6)The expression of NF-?B in BMOV0.25 mg,0.5mg,1mg group,glibenclamide group and diabetic group was higher than that in normal group.BMOV0.25 mg,0.5mg,1mg group,glibenclamide group,diabetes group TNF-? expression was higher than the normal group,the cytoplasm of brown particles increased,of which BMOV0.25 mg of liver cells brownish granules less than diabetic group.The expression of NF-?B and TNF-? increased with the dose of BMOV.Conclusion:1.BMOV at the dose of 0.25 mg/kg·d,0.5 mg/kg·d,1mg/kg·d,intragastric administration for 3 weeks,the blood glucose and blood lipid levels of diabetic rats can not effectively reduce.2.The dose of 0.25mg/kg·d of BMOV can increase the activity of glutathione peroxidase in diabetic rats,decrease the level of malondialdehyde in diabetic rats and improve the antioxidant capacity of diabetic rats.3.The dose of 1mg/kg·d of BMOV,leading to diabetic rats glutathione peroxidase activity levels decreased,increased levels of malondialdehyde,the dose may lead to diabetic rats increased oxidative stress.4.With the test dose of BMOV increased,the degree of liver injury in diabetic rats showed an increasing trend.