| Colorectal cancer(Colorectal cancer,CRC),refers to the human and animal colorectal parts of the malignant tumor,mainly by the intestinal mucosal epithelial cells caused [1].One serious threat to human health,is one of the world's common malignant tumors,located in the world's tumor winning percentage ranked third [2,3] colon cancer early and no obvious symptoms,with the development of the disease,there will be intestinal Pain,blood in the stool,anemia,fever,weight loss,intestinal obstruction,abdominal swelling and other inflammatory symptoms.Late colon cancer patients will appear ascites,edema,jaundice and other metastatic symptoms.Some of the bacteria in the intestinal tract caused by inflammation and colorectal cancer,the relationship between the researchers are increasingly attracted attention.Intestinal flora is also involved in the metabolism of some chemical carcinogens and mutagens in humans,such as acetaldehyde,azo-oxidized methane(AOM),polyamines,phenols,and alkyl compounds [57-59].But some bacteria can play a protective role in the intestinal wall,inhibit the occurrence of colorectal cancer.Based on the above view,the subject of this study to explore the relationship between colon cancer intestinal flora and part of the mechanism of the study.OBJECTIVE: The purpose of this study is to analyze the animal model of colorectal cancer by analyzing the intestinal flora in patients with colorectal cancer and analyze the relationship between colorectal cancer and intestinal microflora,and the related molecular mechanism of colorectal cancer Cancer treatment offers new directions.Contents:(1)collection of clinical colon cancer patients,normal human feces.(2)DMH-induced rat model of colon cancer,DMH-induced CRC rats,was established.(3)To analyze the difference of intestinal flora in colorectal cancer and the difference of intestinal flora in colorectal cancer rats and normal rats to find out the possible pathogens.(4)immunological point of view to verify MUC2 play a role in colon cancer.Methods:(1)Clinical samples of colorectal cancer patients were collected and analyzed by PCR-DGGE method to analyze the difference between colon cancer patients and normal intestinal flora.(2)Anatomical collection of fecal and intestinal tissue samples from experimental rats for pathologic analysis,analysis of intestinal flora and immunological analysis.(3)The establishment of rat colon cancer model was induced by DMH drug.The difference of intestinal flora between colon cancer rats and normal rats was detected by PCR-DGGE technique.(4)combined with clinical colonic cancer patients compared with normal intestinal flora differences,and colon cancer animal model compared to normal intestinal flora differences,found that suspicious colon cancer pathogens.Results:(1)There were significant differences in colorectal cancer between clinical patients and normal control group.(2)The animal model of colon cancer rats was successfully established.There were significant differences in intestinal flora between colon cancer rats and normal rats.(3)To identify one suspicious colon cancer pathogens.The intestinal flora of colon cancer and colon cancer patients induced by carcinogen exist comparability.(4)colon cancer disease on intestinal mucin MUC2 has a down-regulation.Conclusion: Comparing the intestinal microflora structure of rats with colorectal cancer and healthy rats,it is proved that there is a positive correlation between colon cancer and intestinal flora.Downregulation of MUC2 may be involved in the development of colorectal cancer and provide a direction for subsequent clinical diagnosis. |
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