| Objective:Study on the chemical constituents from Tetrastigma planicaul?Hook.?Gagnep.This work can give a scientific basis for ex-posing the antitumor activity extracts from the plant.Methods:T.planicaul was extracted five times by 80%ethanol,and the extract was separated into five parts according to the polarity,including petroleum ether part,ethyl acetate part,n-butanol part and wa-ter part.The constituents were isolated by silicagel,macroporous resin adsorption,polyamide,Sephadex LH-20 chromatography,recrystallization,et al.The structure were identified by spectral analysis(MS,1H-NMR,13C-NMR et al)and comparing with the published data.The active ingr-edient protocatechuic acid of T.planicaul was qualitatively identifiedby TLC,and quantitative dermined by HPLC.Result:Twenty-four compounds were isolated and identified as coelarthenol?1?,ethyl 3?-hydroxy-5-cholen-26-oate?2?,taraxerone?3?,?-sitosterol?4?,daucosterol?5?,protocatechuic acid?6?,dennettine?7?,ethyl gallate?8?,tetradecanoic acid?9?,1-dodecanol?10?,glyceryl pal--mitate?11?,3-??stearyolxy?olean-12-ene?12?,palmitic acid?13?,salicy-lic acid?14?,?-sitosteryl oleate?15?,vanillic acid?16?,stigmasterol?17?,12-methyltridecan-1-ol?18?,?24s?-3?-hydroxy-5?-stigmastan-6-one?19?,isoscopletin?20?,estigmast-4-en-6?-ol-3-one?21?,stigmast-4,2,2-dien-3-one?22?,D-glucose?23?,D-mannitol?24?.Content of protocat--echuic acid of T.planule had a good linearity in the range of 0.02920.584?g?r=0.9998?.The result of methodology validation meets the re-quirement.Conclusion:Compound 13,7,9,10,12,15,1822 are iso-lated from Tetrastigma BI.for the first time.13,7,9,10,12,15,1824 are obtained from this plant for the first time.The method can be used for the estimation of quality of T.planule. |
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