Studies On Hplc Fingerprints?determination And Anti-inflammatory Activity Of Zhuang Medicine Cryptolepis Buchananii Roem. Et Schult.

Objective:Build HPLC fingerprint of stems and leaves from zhuang meficine Cryptolepis buchananii Roem.et Schult.,Take the research of active ingredient chlorogenic acid for the stems and leaves from Cryptolepis buchananii Roem.et Schult.,offers a new way for scientific and effective control of its quality;Analysis and comparison the differences of the extract volatile oil chemical composition for Cryptolepis buchananii Roem.et Schult.to SFE-CO₂ method and SD method;The extracts from different carrying out a research into the acute toxicity and anti-inflammatory activity of Cryptolepis buchananii Roem.et Schult..Methods:?1?Use Thermo ODS-2 Hypersil chromatographic column?5?m,4.6×250mm?,the wavelength of 323 nm,using methanol-phosphoric acid system,gradient elution and chromatogram of each batch of samples using cluster analysis to classify,similarity calculation,will be measured in the same conditions of Cryptolepis buchananii Roem.et Schult.to compared with the stems and leaves maps,analysis the difference.?2?Use acetonitrile phosphate system,such as degree of elution,the wavelength of 329 nm,the active ingredient in stems and leaves of chlorogenic acid content determination.?3?Use SFE-CO₂ method,and SD method to extract the medicinal materials of naphtha respectively,by GC-MS for volatile oil chemical composition on qualitative analysis and comparison.?4?use stems and leaves water extract and alcohol extract of Cryptolepis buchananii Roem.et Schult.the acute toxicity of mice,the result of the foot swelling and cotton ball granuloma in mice acute or chronic inflammation of the experimental study.Result:?1?Determined the stems and leaves HPLC fingerprint of Cryptolepis buchananii Roem.et Schult.,10 batches of Cryptolepis buchananii Roem.et Schult.HPLC fingerprint similarity.?2?In the stems of Cryptolepis buchananii Roem.et Schult.,chlorogenic acid in the linear range of 0.0039 to 0.39 mg/mL,the average recovery was 101.57%,RSD was2.40%?n=6?;In the leaves of Cryptolepis buchananii Roem.et Schult.,chlorogenic acid in the linear range of 0.0039 to 0.39 mg/mL,the average recovery was 99.06%,RSD was 2.10%?n=6?,The 10 batches of chlorogenic acid content determination,methodology,linearity,stability,repeatability,precision and recovery test is good from the stems and leaves of Cryptolepis buchananii Roem.et Schult..?3?SFE-CO₂ method,separation of the 35compounds,28 compounds were identified,accounting for 90.32%of the total outflow components of Cryptolepis buchananii Roem.et Schult.,SD method,separation of the 26 compounds,19 compounds identified,accounting for 95.87%of the total outflow components,including 8 common composition.?4?The water extract and alcohol extract of stems and leaves from Cryptolepis buchananii Roem.et Schult.all have certain toxicity,water extract high dose can significantly reduce the effects of cotton ball induced granulation swelling of mice?P<0.05?,middle and low dose had no significant difference,alcohol extract of high dose could extremely significantly reduce the effects of cotton ball induced granulation swelling of mice?P<0.01?,middle and low dose had no significant difference,the water extract high dose is also extremely significant reduce carrageen glue to toes swelling in mice?P<0.01?,the influence of medium and low dose had significant difference?P<0.05?,alcohol extraction of high,middle dose can significantly reduce the effects of carrageen glue to toes swelling of mice?P<0.01?,there was no significant difference at low doses.Conclusion:Established HPLC fingerprint of stems and leaves from zhuang meficine Cryptolepis buchananii Roem.et Schult.,stems and leaves similarity above 0.926 and 0.888;The stems and leaves in different regions of chlorogenic acid content is different,leaves chlorogenic acid content is obviously higher than stems;SFE-CO₂ and SD method to extract the volatile oil components have certain difference by Cryptolepis buchananii Roem.et Schult..Water and alcohol extracts have certain toxicity and different levels of anti-inflammatory activity.