Association Of CYP2J2 And E-selectin Gene Polymorphisms With Ischemic Stroke And Stroke Subtypes

Part ? Association of CYP2J2 gene polymorphisms with ischemic stroke and Stroke SubtypesObjective: Ischemic stroke is the main cause of mortality and disability among the old people in China and is a multi-factorial disease influenced by many factors including genetic factors like the allele for CYP 2J2.It has been demonstrated that CYP2J2 polymorphisms alter the transcriptional activity.However,studies on the association between CYP2J2-50G/T polymorphism and ischemic stroke have reported conflicting results.Thus,our study aimed to examine the association between four variants in the CYP2J2 gene and the risk of ischemic stroke and its subtypes,in the Chinese population.Materials and Methods: In this study,genotyping was performed by using PCR-sequencing for 202 IS patients and 206 age and sex matched controls.Odds ratios were estimated by multivariate logistic regression and PCR results were confirmed by DNA sequencing.A meta-analysis was conducted to evaluate the association of CYP2J2-50G>T polymorphism with the risk of Ischemic stroke in Chinese population by calculating pooled odds ratio(OR).Results: We found this polymorphism was significantly associated with IS(17.82%vs.10.68%,?2=4.27,P=0.039,OR=1.81,95%CI: 1.03-3.21).Multiple logistic regression analysis revealed that GT genotype was associated with a significantly high risk of ischemic stroke(P=0.011,OR=2.32,95 % CI: 1.21-4.45)after adjustment for other confounding factors such as hypertension,diabetes,hyperlipoidemia,CHD and smoking habit.We also found a significant association of GT genotype with SAA(?2=4.44,P=0.035,OR=2.22,95%CI: 1.04�4.72).Meta-analysis results also showed that the carriers of CYP2J2-50G-T allele had a negative effect on the risk of Ischemic stroke in Chinese with overall OR of 1.40(overall OR =1.40,95 % CI: 1.06�1.84).Conclusion: The findings of the present study suggested that polymorphism in-50G-T position of CYP2J2 gene might be a risk factor for IS in Chinese population.Further large prospective studies were required to confirm these findings.Part ? Association of E-selectin gene polymorphisms with ischemic stroke and stroke subtypesObjective: To investigate the relationship between E-selectin gene polymorphisms and ischemic stroke(IS)in Chinese Han population.Materials and Methods: In our study,genotyping was performed by using PCR-sequencing for 202 IS patients and 206 age and sex matched controls.Two polymorphisms of E-selectin,RS5361 and RS1805193,were genotyped by ABI 3730 automated sequencer and data were analyzed by SPSS 17.0.Results: Statistical difference was detected in the frequency of A and C alleles between cases and controls(?2=11.74,P =0.001,OR=0.07,CI: 0.01-0.52).We also observed distinct difference in the genotypic distribution in two groups(?2=11.96,P=0.001,OR=0.01,CI: 0.01-0.50).In addition,we found a significant association of AC genotypes with large artery atherosclerosis(?2=10.93,P=0.001,OR=0.07,95%CI: 0.01-0.55)and small artery occlusion(?2=15.28,P <0.001,OR=0.05,95%CI: 0.01-0.40).Multiple logistic regression analysis revealed that AC genotype was associated with a significantly high risk of ischemic stroke(P=0.016,OR=13.61,95%CI:1.64-113.44)after adjustment for other confounding factors such as hypertension(P <0.001),diabetes(P <0.001),CHD(P =0.003),smoking habit(P <0.001)and hyperlipoidemia(P <0.001).We didn't find significant statistic difference about the distribution of genotypes and alleles of E-selectin G98 T between the two groups in this study(P>0.05).Conclusion: Gene polymorphism of E-selectin(S128R)was associated with ischemic stroke.The allele of C might be a risk factor for IS in Chinese Han population and individuals bearing AC genotype seem to have a higher propensity to IS than AA genotype.